Genomic cloning of the human histo-blood group ABO locus.

作者信息

Bennett E P, Steffensen R, Clausen H, Weghuis D O, van Kessel A G

机构信息

Faculty of Health Sciences, School of Dentistry, University of Copenhagen, Denmark.

出版信息

Biochem Biophys Res Commun. 1995 Jan 5;206(1):318-25. doi: 10.1006/bbrc.1995.1044.

A P1 phage clone containing the near complete protein coding region of the human histo-blood group ABO gene locus was isolated and characterized. The insert was estimated at approximately 41 kp, and an approximately 20 kbp BamHI fragment contained the near complete coding region as evaluated by Southern blot analysis. Intron/exon boundaries were determined and the coding region was found to span at least seven exons. In situ hybridization to metaphase chromosomes confirmed the predicted localization to 9q34.