Kawaguchi M, Kawashima F, Ohshima K, Kawaguchi S, Wada H
Department of Pediatrics, Hyogo College of Medicine, Nishinomiya, Japan.
Pathobiology. 1994;62(5-6):283-91. doi: 10.1159/000163921.
Glomerular epithelial cells (GEC) in culture facilitate the study of glomerular physiology and pathology. However, characterization and identification of GECs in culture have been difficult due to the absence of markers specific to them. We compared the immunocytochemical characteristics of a rat normal GEC line (SGE1) and glomerular cells from rat kidney sections using a lectin and commercially available and newly raised monoclonal and polyclonal antibodies. Antivimentin, anti-dipeptidyl-peptidase-IV (gp 108) and monoclonal antibody 5-1-6 antibodies and Limax flavus lectin bound to visceral GECs, anticytokeratin antibody and antibody against common acute lymphocytic leukemia antigen bound to parietal GECs, and anti-SGE1 cell membrane and monoclonal antibody PHM 5 antibodies bound to both visceral GECs and parietal GECs in normal rat kidney sections, and all of these antibodies and L. flavus lectin consistently bound to SGE1 cells in culture. The pattern of antigenic expression on SGE1 cells indicates that SGE1 cells possess phenotypic characteristics of visceral GECs and parietal GECs, and it further suggests that SGE1 cells may be stem cells or cells undergoing differentiation.
培养的肾小球上皮细胞（GEC）有助于肾小球生理学和病理学的研究。然而，由于缺乏特异性标记物，培养的GEC的表征和鉴定一直很困难。我们使用一种凝集素以及市售的和新制备的单克隆和多克隆抗体，比较了大鼠正常GEC系（SGE1）和大鼠肾切片中肾小球细胞的免疫细胞化学特征。抗波形蛋白、抗二肽基肽酶-IV（gp 108）和单克隆抗体5-1-6抗体以及黄蛞蝓凝集素与脏层GEC结合，抗细胞角蛋白抗体和抗常见急性淋巴细胞白血病抗原抗体与壁层GEC结合，抗SGE1细胞膜抗体和单克隆抗体PHM 5抗体在正常大鼠肾切片中与脏层GEC和壁层GEC均结合，并且所有这些抗体和黄蛞蝓凝集素在培养中均持续与SGE1细胞结合。SGE1细胞上的抗原表达模式表明，SGE1细胞具有脏层GEC和壁层GEC的表型特征，并且进一步表明SGE1细胞可能是干细胞或正在分化的细胞。
