Schober A, Walter N G, Tangen U, Strunk G, Ederhof T, Dapprich J, Eigen M
Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
Biotechniques. 1995 Apr;18(4):652-61.
As an improved strategy for producing functional macromolecules by in vitro evolutionary optimization, we propose an automated machine that can process up to 960 samples in parallel. It consists of a 960-well PCR machine with special sealed plastic reaction vessels and appropriate handling devices. We show that the heat-sealing technique does not significantly affect the activity of Taq DNA Polymerase or that of the temperature-sensitive Q beta RNA polymerase but avoids cross-contamination and evaporation of the samples. Initial experiments demonstrate the suitability of the apparatus to uniformly process the samples and to perform the thermocycling. Serial transfer of reaction products into fresh reaction solution was used to initiate further rounds of amplification as a typical experimental setup for an evolutionary biotechnology application.
作为一种通过体外进化优化来生产功能性大分子的改进策略，我们提出了一种能够并行处理多达960个样本的自动化机器。它由一台带有特殊密封塑料反应容器和适当处理装置的960孔PCR仪组成。我们表明，热封技术不会显著影响Taq DNA聚合酶或温度敏感的Qβ RNA聚合酶的活性，但能避免样本的交叉污染和蒸发。初步实验证明了该仪器适用于均匀处理样本并进行热循环。将反应产物连续转移到新鲜反应溶液中以启动进一步的扩增轮次，这是进化生物技术应用的典型实验设置。
