基质及其化学活化对免疫吸附剂稳定性的重要性。
Importance of the matrix and its chemical activation on the stability of immuno-adsorbents.
作者信息
Ubrich N, Hubert P, Dellacherie E, Rivat C
机构信息
Centre de recherche INSERM, CHU Brabois, France.
出版信息
J Mol Recognit. 1995 Jan-Apr;8(1-2):111-5. doi: 10.1002/jmr.300080120.
Various matrices were reacted with four different activation reagents, in order to prepare immuno-adsorbents for the selective removal of low-density lipoproteins from blood plasma of patients affected by hypercholesterolaemia. The resulting immuno-adsorbents were compared to that obtained earlier with cyanogen bromide activation, in terms of coupling yield, adsorption capacity and moreover stability towards the various chemical and biochemical conditions to which they are submitted during their handling. Tresyl chloride-activated Sepharose 6 Fast Flow turns out to afford optimal stability in the whole pH range tested.
将各种基质与四种不同的活化试剂反应，以制备免疫吸附剂，用于从高胆固醇血症患者的血浆中选择性去除低密度脂蛋白。将所得的免疫吸附剂与早期通过溴化氰活化获得的免疫吸附剂在偶联产率、吸附容量以及在处理过程中所经受的各种化学和生化条件下的稳定性方面进行比较。结果表明，经三氟甲磺酸酰氯活化的琼脂糖凝胶6快速流动型在整个测试pH范围内具有最佳稳定性。
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