McCarty L P, Karr S M, Harris B Z, Michelson S G, Leith J T
Department of Radiation Medicine, Brown University School of Medicine, Providence, Rhode Island 02912, USA.
Br J Cancer. 1995 Jul;72(1):10-6. doi: 10.1038/bjc.1995.269.
We measured levels of basic fibroblast growth factor (FGF-2) in human colon cancer cells (clone A) in vitro and in xenografted solid tumours using a commercial enzyme-linked immunoassay. In Vitro, levels in unfed plateau phase or exponentially growing cells were low, averaging respectively about 2 and 8 pg 10(-6) cells. However, when solid tumours (average volumes 787 mm3) were cut into halves and either enzymatically disaggregated to obtain a cellular fraction or extracted in toto, levels were much higher. In the cellular fraction, values averaged 110 pg 10(-6) cells, while in whole tumour extracts, average values were 24 pg mg-1 tumour tissue. These results indicate that growth factor levels in solid neoplasms may differ markedly from those predicted from in vitro measurements. We hypothesise that the apparent increase in FGF-2 levels in vivo results primarily from the presence of a significant fraction of host cells (in particular, macrophages, which may contain high levels of FGF-2) within xenografted clone A neoplasms.
我们使用商用酶联免疫测定法，在体外对人结肠癌细胞（克隆A）以及在异种移植实体瘤中测量碱性成纤维细胞生长因子（FGF-2）的水平。在体外，处于未喂食平台期或指数生长期的细胞中，FGF-2水平较低，平均分别约为2 pg/10⁻⁶个细胞和8 pg/10⁻⁶个细胞。然而，当将实体瘤（平均体积787 mm³）切成两半，要么通过酶解获得细胞部分，要么整体提取时，FGF-2水平要高得多。在细胞部分，平均含量为110 pg/10⁻⁶个细胞，而在整个肿瘤提取物中，平均值为24 pg/mg肿瘤组织。这些结果表明，实体瘤中的生长因子水平可能与体外测量预测的水平有显著差异。我们推测，体内FGF-2水平的明显升高主要是由于异种移植的克隆A肿瘤中存在相当一部分宿主细胞（特别是巨噬细胞，其可能含有高水平的FGF-2）。
