体外培养的A克隆人结肠癌细胞中碱性成纤维细胞生长因子水平与异种移植肿瘤中该因子水平的比较。
Comparison of basic fibroblast growth factor levels in clone A human colon cancer cells in vitro with levels in xenografted tumours.
作者信息
McCarty L P, Karr S M, Harris B Z, Michelson S G, Leith J T
机构信息
Department of Radiation Medicine, Brown University School of Medicine, Providence, Rhode Island 02912, USA.
出版信息
Br J Cancer. 1995 Jul;72(1):10-6. doi: 10.1038/bjc.1995.269.
We measured levels of basic fibroblast growth factor (FGF-2) in human colon cancer cells (clone A) in vitro and in xenografted solid tumours using a commercial enzyme-linked immunoassay. In Vitro, levels in unfed plateau phase or exponentially growing cells were low, averaging respectively about 2 and 8 pg 10(-6) cells. However, when solid tumours (average volumes 787 mm3) were cut into halves and either enzymatically disaggregated to obtain a cellular fraction or extracted in toto, levels were much higher. In the cellular fraction, values averaged 110 pg 10(-6) cells, while in whole tumour extracts, average values were 24 pg mg-1 tumour tissue. These results indicate that growth factor levels in solid neoplasms may differ markedly from those predicted from in vitro measurements. We hypothesise that the apparent increase in FGF-2 levels in vivo results primarily from the presence of a significant fraction of host cells (in particular, macrophages, which may contain high levels of FGF-2) within xenografted clone A neoplasms.
我们使用商用酶联免疫测定法，在体外对人结肠癌细胞（克隆A）以及在异种移植实体瘤中测量碱性成纤维细胞生长因子（FGF-2）的水平。在体外，处于未喂食平台期或指数生长期的细胞中，FGF-2水平较低，平均分别约为2 pg/10⁻⁶个细胞和8 pg/10⁻⁶个细胞。然而，当将实体瘤（平均体积787 mm³）切成两半，要么通过酶解获得细胞部分，要么整体提取时，FGF-2水平要高得多。在细胞部分，平均含量为110 pg/10⁻⁶个细胞，而在整个肿瘤提取物中，平均值为24 pg/mg肿瘤组织。这些结果表明，实体瘤中的生长因子水平可能与体外测量预测的水平有显著差异。我们推测，体内FGF-2水平的明显升高主要是由于异种移植的克隆A肿瘤中存在相当一部分宿主细胞（特别是巨噬细胞，其可能含有高水平的FGF-2）。
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