人肝脏亚硫酸盐氧化酶的分子克隆
Molecular cloning of human liver sulfite oxidase.
作者信息
Garrett R M, Bellissimo D B, Rajagopalan K V
机构信息
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
出版信息
Biochim Biophys Acta. 1995 Jun 9;1262(2-3):147-9. doi: 10.1016/0167-4781(95)00068-r.
PMID:7599189
Abstract
A 2.4 kilobase cDNA clone of human sulfite oxidase was isolated from a human liver cDNA library in lambda gt10. Comparison of three sulfite oxidase sequences to several plant and fungal nitrate reductase sequences reveals a single conserved cysteine with highly conserved flanking sequences. The conserved cysteine is postulated to be a ligand of molybdenum in sulfite oxidase and nitrate reductase.
摘要
从λgt10载体中的人肝脏cDNA文库中分离出一个2.4千碱基对的人亚硫酸盐氧化酶cDNA克隆。将三个亚硫酸盐氧化酶序列与几种植物和真菌的硝酸还原酶序列进行比较，发现一个单一的保守半胱氨酸，其侧翼序列高度保守。推测这个保守的半胱氨酸是亚硫酸盐氧化酶和硝酸还原酶中钼的配体。
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