Zentay Z, Raguwanshi M, Reddi A, Lasker N, Dasmahapatra A, Aviv A
Hypertension Research Center, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark 07103-2714, USA.
J Diabetes Complications. 1995 Apr-Jun;9(2):74-80. doi: 10.1016/1056-8727(94)00007-b.
In this study, human platelets were used as a cellular model for exploring cytosolic free Ca (Cai) regulation in non-insulin-dependent diabetes mellitus (NIDDM). Cai levels were monitored in resting and thrombin-stimulated platelets from obese females with NIDDM; obese, nondiabetic women, and nonobese, nondiabetic women. All subjects were black. Significant and marked elevation of basal Cai levels was observed in platelets from the diabetic subjects when no aspirin was used during platelet isolation. However, no significant differences were observed in Cai between aspirin-treated platelets from women with NIDDM and platelets from nondiabetic women. The rate of the Cai return to basal level after thrombin stimulation was significantly lower in platelets from the diabetic subjects, suggesting an abnormality in platelet Ca extrusion or sequestration in NIDDM. Platelet Cai levels positively correlated with low-density lipoprotein cholesterol/high-density lipoprotein cholesterol ratio (LDL/HDL) and fasting blood glucose. These findings suggest abnormalities in platelet Cai homeostasis in NIDDM that are influenced by the serum lipid profile and perhaps glucose.
在本研究中，人血小板被用作细胞模型，以探索非胰岛素依赖型糖尿病（NIDDM）中细胞溶质游离钙（Cai）的调节。监测了患有NIDDM的肥胖女性、肥胖非糖尿病女性和非肥胖非糖尿病女性静息和凝血酶刺激血小板中的Cai水平。所有受试者均为黑人。在血小板分离过程中未使用阿司匹林时，糖尿病受试者血小板中的基础Cai水平显著且明显升高。然而，NIDDM女性经阿司匹林处理的血小板与非糖尿病女性血小板的Cai水平无显著差异。凝血酶刺激后，糖尿病受试者血小板中Cai恢复到基础水平的速率显著较低，表明NIDDM中血小板钙的外排或螯合存在异常。血小板Cai水平与低密度脂蛋白胆固醇/高密度脂蛋白胆固醇比值（LDL/HDL）和空腹血糖呈正相关。这些发现表明，NIDDM中血小板Cai稳态存在异常，受血清脂质谱以及可能的葡萄糖影响。
