Logothetou-Rella H
Department of Experimental Physiology, Medical School, University of Athens, Greece.
Histol Histopathol. 1995 Apr;10(2):271-82.
In situ cytogenetic morphology and analysis showed that Epstein-Barr Virus (EBV)-infected Raji and EBV-producing P3HR-1 cells divide by meiosis and follow the life cycle of malignant cells in vitro. Meiosis was documented by the presence of condensed chromosomes, "o" chromosome, nuclear vlimata (NVs), NV invasion, extrusion of chromosomes, chromosomal transfer, metaphase fusion and aneuploidy. EBV-Raji, EBV-producing P3HR, HIV1-infected MOLT-4 cells (dividing by meiosis) and human spermatozoa (cellular products of meiosis) were highly sensitive to the endogenous inhibitor (CANP-I) of calcium-activated neutral proteinase (CANP). CANP-I-treated virally infected and virus producing cells showed necrosis and disappearance of immunofluorescent viral antigens, documenting the anti-viral action of CANP-I. CANP-I-treated spermatozoa exhibited arrested motility with subsequent necrosis documenting the in vitro spermicidal action of CANP-I. The testis of treated Wistar rats with 0.25 U CANP-I/gr body weight for six days, were devoid of spermatozoa, with pronounced toxicity and exfoliation of spermatocytes and spermatids, indicating the contraceptive action of CANP-I via spermatogenetic arrest. It is concluded that CANP-I is an anti-meiotic agent inhibiting CANP associated with meiosis. Hence CANP-I is a promising agent against various diseases involving meiosis.
原位细胞遗传学形态学及分析表明，爱泼斯坦 - 巴尔病毒（EBV）感染的拉吉细胞和产生EBV的P3HR - 1细胞在体外通过减数分裂进行增殖，并遵循恶性细胞的生命周期。减数分裂通过浓缩染色体、“o”染色体、核包膜（NVs）、NV入侵、染色体挤出、染色体转移、中期融合及非整倍体的存在得以证实。EBV - 拉吉细胞、产生EBV的P3HR细胞、感染HIV1的MOLT - 4细胞（通过减数分裂进行增殖）及人类精子（减数分裂的细胞产物）对钙激活中性蛋白酶（CANP）的内源性抑制剂（CANP - I）高度敏感。经CANP - I处理的病毒感染细胞和产生病毒的细胞出现坏死，免疫荧光病毒抗原消失，证明了CANP - I的抗病毒作用。经CANP - I处理的精子活力停滞，随后坏死，证明了CANP - I的体外杀精作用。用0.25 U CANP - I/克体重处理Wistar大鼠6天，其睾丸中无精子，出现明显毒性，精母细胞和精子细胞脱落，表明CANP - I通过抑制精子发生起到避孕作用。结论是，CANP - I是一种抑制与减数分裂相关的CANP的抗减数分裂剂。因此，CANP - I是一种有前景的针对各种涉及减数分裂疾病的药物。
