Tsuji J M, Pollack S B
Department of Biological Structure, University of Washington, Seattle 98195, USA.
Nat Immun. 1995 Jan-Feb;14(1):44-56.
To test whether differentiation of natural killer (NK) precursor cells in unsupplemented long-term bone marrow culture requires contact with stromal cells, bone marrow cells (BMC) that were depleted of NK 1.1+ and Thy-1+ cells were cultured either directly on established, irradiated bone marrow stroma or in cell culture inserts separated from the stroma by a 0.45-micron microporous membrane. No exogenous cytokines were added. Depleted BMC produced NK 1.1+ nonlytic cells by day 7 and lytic NK 1.1+ cells by day 14 only when grown directly on the stroma. NK precursor cells survived in the inserts, however, and could respond to recombinant interleukin-2 in secondary culture. Thus, stromal cell-dependent proliferation and differentiation of lytic NK 1.1+ cells from their nonlytic NK 1.1- precursors requires direct interaction between stromal cells and NK precursors.
为了检测在未添加补充物的长期骨髓培养中自然杀伤（NK）前体细胞的分化是否需要与基质细胞接触，将去除了NK 1.1⁺和Thy-1⁺细胞的骨髓细胞（BMC）直接培养在已建立的经辐照的骨髓基质上，或者培养在通过0.45微米微孔膜与基质分离的细胞培养插入物中。未添加外源性细胞因子。仅当直接生长在基质上时，去除的BMC在第7天产生NK 1.1⁺非裂解细胞，在第14天产生裂解性NK 1.1⁺细胞。然而，NK前体细胞在插入物中存活，并且在二次培养中能够对重组白细胞介素-2作出反应。因此，从其非裂解性NK 1.1⁻前体中产生裂解性NK 1.1⁺细胞的基质细胞依赖性增殖和分化需要基质细胞与NK前体之间的直接相互作用。
