Hill C S, Wynne J, Treisman R
Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London, England.
Cell. 1995 Jun 30;81(7):1159-70. doi: 10.1016/s0092-8674(05)80020-0.
The c-fos serum response element (SRE) forms a ternary complex with the transcription factors SRF (serum response factor) and TCF (ternary complex factor). By itself, SRF can mediate transcriptional activation induced by serum, lysophosphatidic acid, or intracellular activation of heterotrimeric G proteins. Activated forms of the Rho family GTPases RhoA, Rac1, and CDC42Hs also activate transcription via SRF and act synergistically at the SRE with signals that activate TCF. Functional Rho is required for signaling to SRF by several stimuli, but not by activated CDC42Hs or Rac1. Activation of the SRF-linked signaling pathway does not correlate with activation of the MAP kinases ERK, SAPK/JNK, or MPK2/p38. Functional Rho is required for regulated activity of the c-fos promoter. These results establish SRF as a nuclear target of a novel Rho-mediated signaling pathway.
c-fos血清反应元件（SRE）与转录因子血清反应因子（SRF）和三元复合因子（TCF）形成三元复合物。单独来看，SRF可介导由血清、溶血磷脂酸或异源三聚体G蛋白的细胞内激活所诱导的转录激活。Rho家族GTP酶RhoA、Rac1和CDC42Hs的激活形式也通过SRF激活转录，并在SRE处与激活TCF的信号协同作用。几种刺激向SRF发出信号需要功能性Rho，但激活的CDC42Hs或Rac1则不需要。与SRF相关的信号通路的激活与丝裂原活化蛋白激酶ERK、应激激活蛋白激酶/JNK或丝裂原活化蛋白激酶2/p38的激活无关。c-fos启动子的调节活性需要功能性Rho。这些结果确立了SRF作为一种新型Rho介导信号通路的核靶点。
