Numasaki M, Fukuoka Y, Kudo T, Saeki H, Tachibana T, Motomiya M, Nukiwa T
Department of Respiratory Medicine, Tohoku University, Sendai, Japan.
Int J Cancer. 1995 Jul 4;62(1):42-7. doi: 10.1002/ijc.2910620110.
Mononuclear cells from the peripheral blood of patients with systemic lupus erythematosus (SLE) were transformed with the Epstein-Barr virus (EBV) and the resultant polyclonal B-lymphoblastoid cell lines were tested for antibody activity to membrane antigens of certain T-cell lines. B lymphoblastoid cell lines secreting specific antibodies were fused with (mouse x human) heteromyeloma SHM-D33 cells. Among the large number of hybridomas generated, one which produced a human monoclonal antibody (MAb) TONO-1 (IgM, lambda) was selected. MAb TONO-1 proved to be reactive with 4 human T-cell lines, HPB-MLT, L-MAT, MOLT-3 and MOLT-4F, but not with B-leukemia, Burkitt's lymphoma, myelomonocytic leukemia, erythroleukemia or non-hematopoietic malignant cell lines. MAb TONO-1 reacted positively with fresh leukemia cells from 2 of 7 patients with acute T-lymphocytic leukemia, but no reaction was observed in non-T-cell leukemia cases. Normal lymphocytes, monocytes, granulocytes, red blood cells and platelets in the peripheral blood did not demonstrate remarkable binding. Neither thymocytes nor bone-marrow cells from healthy volunteers were reactive. The antigens defined by MAb TONO-1 were polypeptides of 57 kDa and 68 kDa. Immunohistological studies revealed no staining of thymocytes in the thymus of a 6-month-old child, but showed epithelial reticular cells and Hassall's corpuscles to stain positively. These results suggest that MAb TONO-1 is directed to T-leukemic cells and some components of thymus tissue.
用爱泼斯坦-巴尔病毒（EBV）转化系统性红斑狼疮（SLE）患者外周血中的单核细胞，并检测由此产生的多克隆B淋巴母细胞系对某些T细胞系膜抗原的抗体活性。将分泌特异性抗体的B淋巴母细胞系与（小鼠×人）杂种骨髓瘤SHM-D33细胞融合。在产生的大量杂交瘤中，选择了一种产生人单克隆抗体（MAb）TONO-1（IgM，λ）的杂交瘤。MAb TONO-1被证明与4个人T细胞系HPB-MLT、L-MAT、MOLT-3和MOLT-4F反应，但与B淋巴细胞白血病、伯基特淋巴瘤、骨髓单核细胞白血病、红白血病或非造血恶性细胞系无反应。MAb TONO-1与7例急性T淋巴细胞白血病患者中2例的新鲜白血病细胞呈阳性反应，但在非T细胞白血病病例中未观察到反应。外周血中的正常淋巴细胞、单核细胞、粒细胞、红细胞和血小板未显示明显结合。健康志愿者的胸腺细胞和骨髓细胞均无反应性。MAb TONO-1定义的抗原是57 kDa和68 kDa的多肽。免疫组织学研究显示，6个月大儿童胸腺中的胸腺细胞无染色，但上皮网状细胞和哈索尔小体呈阳性染色。这些结果表明，MAb TONO-1针对T白血病细胞和胸腺组织的某些成分。
