Hibbard B, Moseley W M, Robinson J A, Boucher J F
Upjohn Company, Kalamazoo, MI 49001, USA.
J Anim Sci. 1995 Feb;73(2):526-33. doi: 10.2527/1995.732526x.
In a randomized complete block design with repeated measures, ruminally cannulated crossbred beef steers (n = 24; 279 +/- 3.2 kg) had ad libitum access to a 90% concentrate diet and were injected daily with slaframine dichloride (SF) at doses of 0, 30, or 60 micrograms SF free-base/kg BW. On d 1, 2, 7, 14, and 21, salivary scores were determined hourly from -2 to 8 h relative to injection (0 h), and ruminal pH was measured at 0, 4, and 8 h. Feed intake was measured daily. Serum concentrations of somatotropin (ST) were measured in samples collected at 20-min intervals from -40 min to 8 h on d 2, 7, 14, and 21, and IGF-I was measured at 8 h. Mean salivary score increased with SF injection (P < .01; .4, 3.3, and 3.7 for 0, 30, and 60 micrograms of SF/kg BW, respectively), although there was a dose x day interaction (P < .01). Injection of SF reduced DMI (P < or = .01; 6.39, 4.95, and 2.64 kg of DM/d for 0, 30, and 60 micrograms of SF/kg BW, respectively). Mean ruminal pH was increased (P < .01); 6.03, 6.42, and 6.72 for 0, 30, and 60 micrograms of SF/kg BW, respectively). Steers administered 60 micrograms of SF/kg BW lost weight (P = .02; 15.1 and -20.3 kg for 0 and 60 micrograms of SF/kg BW, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)
在一项采用重复测量的随机完全区组设计中，瘤胃插管的杂交肉牛（n = 24；体重279±3.2千克）可自由采食90%精料日粮，每天按0、30或60微克游离碱形式的二氯司拉明（SF）/千克体重的剂量注射SF。在第1、2、7、14和21天，在相对于注射时间（0小时）的-2至8小时内每小时测定唾液评分，并在0、4和8小时测量瘤胃pH值。每天测量采食量。在第2、7、14和21天，从-40分钟至8小时每隔20分钟采集的样本中测量生长激素（ST）的血清浓度，并在8小时测量胰岛素样生长因子-I（IGF-I）。尽管存在剂量×天数的交互作用（P <.01），但唾液评分均值随SF注射量增加（P <.01；0、30和60微克SF/千克体重时分别为0.4、3.3和3.7）。注射SF降低了干物质采食量（DMI）（P≤.01；0、30和60微克SF/千克体重时分别为6.39、4.95和2.64千克干物质/天）。瘤胃pH值均值升高（P <.01）；0、30和60微克SF/千克体重时分别为6.03、6.42和6.72。按60微克SF/千克体重给药的肉牛体重减轻（P =.02；0和60微克SF/千克体重时分别为15.1千克和-20.3千克）。（摘要截选至250字）
