Preus H R, Lassen J, Aass A M, Ciancio S G
Department of Periodontology, School of Dentistry, University of Oslo, Norway.
J Clin Periodontol. 1995 May;22(5):380-4. doi: 10.1111/j.1600-051x.1995.tb00164.x.
The aim of the present study was to compare total numbers of cultivable bacteria and prevalence of resistance to minocycline among periodontal bacteria following subgingival or systemic application of minocycline in patients suffering from periodontal disease. 10 adult patients were administered 2% minocycline ointment subgingivally into their periodontal pockets at baseline, week 2 and months 1, 3, 6 and 9. Patients had scaling/root planing at baseline and month 6. In addition, 10 patients undergoing scaling/root planing followed by a 10-day course of systemic minocycline therapy, were studied and compared with the subgingival application group. Bacterial samples were taken from the 4 deepest pockets before each subgingival application of the drug. The systemic administration group was sampled at baseline and at week 2, as well as months 1 and 3 after completing the antibiotic treatment. For each patient at each sampling, bacterial samples were pooled, diluted, seeded on parallel blood agar plates and incubated aerobically and anerobically. After incubation, 30 colonies were picked at random and transferred to blood agar plates supplemented with 10 micrograms/ml minocycline, to estimate prevalence of minocycline-resistant bacteria. The results of this study indicate that subgingival application of minocycline ointment resulted in an initial reduction in total numbers of cultivable bacteria, which then remained depressed during the full year of the study. No such observation was made in the systemic administration. Both in the subgingival and the systemic administration group, the % of cultivable aerobic and anaerobic minocycline-resistant bacterial strains increased transiently following administration of the drug, but returned to baseline levels within 3 months post-treatment.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是比较在牙周病患者中，龈下或全身应用米诺环素后，牙周细菌中可培养细菌的总数以及对米诺环素的耐药率。10名成年患者在基线、第2周以及第1、3、6和9个月时，龈下接受2%米诺环素软膏治疗，涂抹于牙周袋内。患者在基线和第6个月时进行了龈上洁治/根面平整。此外，研究了10名接受龈上洁治/根面平整并随后进行为期10天全身米诺环素治疗的患者，并与龈下应用组进行比较。在每次龈下应用药物前，从4个最深的牙周袋中采集细菌样本。全身给药组在基线、第2周以及完成抗生素治疗后的第1和3个月进行采样。对于每个患者每次采样，将细菌样本混合、稀释，接种在平行的血琼脂平板上，分别进行需氧和厌氧培养。培养后，随机挑选30个菌落，转移至添加有10微克/毫升米诺环素的血琼脂平板上，以评估耐米诺环素细菌的比例。本研究结果表明，龈下应用米诺环素软膏导致可培养细菌总数最初减少，在研究的一整年中一直保持较低水平。全身给药组未观察到这种情况。在龈下和全身给药组中，给药后可培养的需氧和厌氧耐米诺环素细菌菌株的百分比均短暂增加，但在治疗后3个月内恢复至基线水平。（摘要截短至250字）
