Morsy T A, el Missiry A G, Sarwat M A, el Rassed M M, Habib F S, Abou Gamra M M
Department of Parasitology, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, Egypt.
J Egypt Soc Parasitol. 1995 Apr;25(1):31-51.
Generally speaking, the cell mediated immunity plays a pivotal role in the host resistance against the protozoal parasite of the genus Leishmania. The polypeptide hormone cachectin (Tumour Necrosis Factor-alpha) which is produced mainly by macrophages, is a primary mediator in the pathogenesis of infection, injury and inflammation. Depending on its concentration, duration of cell exposure and presence of other mediators in the cellular environment, the net biological effect of this peptide regulatory factor may ultimately be benefit or injury to the host. This work aimed to study the tumour necrosis factor-alpha as measured by Quantikine human immunoassay Kits using ELISA technique in Egyptian visceral leishmaniasis patients (VL). The severity of VL. was measured by IHAT Cellognost Kits. The results showed a certain type of correlation between elevated serum TNF-alpha and human visceral leishmaniasis. It was concluded that TNF-a is an important factor in the pathogenesis of visceral leishmaniasis and may be a marker or an indicator of the VL. activity.
一般来说，细胞介导的免疫在宿主抵抗利什曼原虫属原生动物寄生虫的过程中起着关键作用。主要由巨噬细胞产生的多肽激素恶病质素（肿瘤坏死因子-α）是感染、损伤和炎症发病机制中的主要介质。根据其浓度、细胞暴露持续时间以及细胞环境中其他介质的存在情况，这种肽调节因子的净生物学效应最终可能对宿主有益或有害。这项工作旨在研究使用酶联免疫吸附测定（ELISA）技术通过Quantikine人免疫测定试剂盒检测埃及内脏利什曼病患者（VL）中的肿瘤坏死因子-α。VL的严重程度通过IHAT Cellognost试剂盒进行测量。结果显示血清肿瘤坏死因子-α升高与人类内脏利什曼病之间存在某种类型的相关性。得出的结论是，肿瘤坏死因子-α是内脏利什曼病发病机制中的一个重要因素，可能是VL活动的一个标志物或指标。
