Amiel D, Harwood F L, Gelberman R H, Chu C R, Seiler J G, Abrahamsson S
Department of Orthopaedics, University of California, San Diego, La Jolla 92093-0630, USA.
J Orthop Res. 1995 May;13(3):459-63. doi: 10.1002/jor.1100130321.
On the basis of recent evidence that the healing processes of tendon grafts are donor-tissue specific, in situ hybridization, using a 372 bp cDNA fragment complementary to a portion of pro alpha 1(I) collagen mRNA, was utilized to compare the cellular responses to transplantation exhibited by autogenous intrasynovial and extrasynovial flexor tendon grafts. Intrasynovial and extrasynovial tendons from the hindpaw were transferred to synovial sheaths in the forepaw of 12 mongrel dogs (24 tendons) and treated with immediate controlled passive motion. The tendon grafts were harvested at 2, 4, and 6 weeks, and each was divided into a proximal, central (8 mm), and distal portion. Sections from the central portion were embedded in paraffin and subjected to in situ hybridization, autoradiography, and staining; levels of procollagen mRNA then were assessed by microscopic examination. The two types of tendon grafts exhibited different levels of pro alpha 1(I) collagen mRNA expression at all three time points. Intrasynovial tendon grafts displayed no areas of increased type-I procollagen mRNA at 2, 4, and 6 weeks. The extrasynovial tendon grafts displayed increased surface levels of type-I procollagen mRNA at 2 and 4 weeks; the levels decreased to background levels by 6 weeks. The high levels of procollagen mRNA exhibited by the extrasynovial grafts suggest increased collagen synthetic activity, indicative of a cellular response to injury, whereas the preservation of low levels of expression in the intrasynovial grafts may signify a less inflammatory cellular response.
基于近期有关肌腱移植物愈合过程具有供体组织特异性的证据，本研究利用原位杂交技术，采用与proα1(I)胶原mRNA一部分互补的372 bp cDNA片段，比较自体滑膜内和滑膜外屈肌腱移植物对移植所表现出的细胞反应。将来自后爪的滑膜内和滑膜外肌腱转移至12只杂种犬（24条肌腱）前爪的滑膜鞘中，并进行即时控制性被动活动治疗。在2周、4周和6周时采集肌腱移植物，每根移植物均分为近端、中间（8 mm）和远端部分。将中间部分的切片嵌入石蜡，进行原位杂交、放射自显影和染色；然后通过显微镜检查评估前胶原mRNA水平。在所有三个时间点，两种类型的肌腱移植物均表现出不同水平的proα1(I)胶原mRNA表达。滑膜内肌腱移植物在2周、4周和6周时均未显示I型前胶原mRNA增加的区域。滑膜外肌腱移植物在2周和4周时显示I型前胶原mRNA表面水平增加；到6周时，这些水平降至背景水平。滑膜外移植物中高水平的前胶原mRNA表明胶原合成活性增加，这是对损伤的细胞反应的指标，而滑膜内移植物中低水平表达的维持可能意味着炎症细胞反应较少。
