Boldt-Houle D M, Nigida S M, Rinaldo C R, Ehrlich G D
Department of Pathology, University of Pittsburgh School of Medicine, PA 15261, USA.
J Med Primatol. 1994 Oct;23(8):432-41. doi: 10.1111/j.1600-0684.1994.tb00132.x.
Using a panel of human T-cell receptor (TCR) variable region beta chain (V beta) polymerase chain reaction (PCR) primers, we performed cross-sectional and longitudinal analyses of the TCR V beta repertoire in naive and HIV-1 infected chimpanzees. We demonstrate that our TCR PCR primer panel will support amplification of chimpanzee cDNA from most of the TCR V beta families. However, no differences in TCR V beta expression were found between the naive and HIV-1 infected chimpanzees, unlike the TCR V beta repertoire perturbation found in HIV-1 infected human subjects. This finding suggests that a complete TCR repertoire in HIV-1 infected chimpanzees is associated with the maintenance CD4+ T-cell numbers and lack of progression to AIDS.
我们使用一组人T细胞受体（TCR）可变区β链（Vβ）聚合酶链反应（PCR）引物，对未感染和感染HIV-1的黑猩猩的TCR Vβ库进行了横断面和纵向分析。我们证明，我们的TCR PCR引物组能够支持从大多数TCR Vβ家族中扩增黑猩猩cDNA。然而，与在感染HIV-1的人类受试者中发现的TCR Vβ库扰动不同，在未感染和感染HIV-1的黑猩猩之间未发现TCR Vβ表达差异。这一发现表明，感染HIV-1的黑猩猩中完整的TCR库与CD4+ T细胞数量的维持以及未进展至艾滋病有关。
