Yao Y M, Tian H M, Sheng Z Y, Wang Y P, Yu Y, Sun S R, Xu S H
Trauma Center, Postgraduate Medical College, 304th Hospital, Beijing, People's Republic of China.
J Trauma. 1995 Jun;38(6):924-30. doi: 10.1097/00005373-199506000-00018.
The current experiments were performed to determine the effects of a subtherapeutic dose of polymyxin B sulfate on gut origin endotoxemia/bacterial translocation, and tumor necrosis factor (TNF) and interleukin-1 (IL-1) release following hemorrhagic shock (30 mm Hg, 90 min) in rats. The results showed that significant portal and systemic endotoxemia took place in the control group (portal, 0.269 to 0.845 endotoxin units (EU)/mL; systemic, 0.164 to 0.655 EU/mL), but not in the treatment group (except 0.5 hour in portal blood: 0.207 +/- 0.094 EU/mL). Concomitantly, the incidence of bacterial translocation to the mesenteric lymph nodes and viscera were reduced significantly at 0.5, 2, 6, and 24 hours postresuscitation in animals receiving polymyxin B (p < 0.05 to 0.01), whereas there were no differences with respect to number of translocating bacteria between the two groups (p > 0.05). Marked elevation of plasma TNF levels and IL-1 activities of peritoneal macrophages were also found in untreated controls at 0.5 to 2 hours (p < 0.05) and 6 to 24 hours (p < 0.05 to 0.01), respectively, but prevented by administration of low-dose polymyxin B. The 48-hour survival rate was improved from 41.7% in the control group to 75.0% in the treatment ones (p > 0.05). These data suggest that pretreatment with a subtherapeutic dose of polymyxin B is effective to inhibit hemorrhage-induced endotoxin/bacterial translocation from the gut and excessive TNF and IL-1 production.
进行当前实验以确定亚治疗剂量的硫酸多粘菌素B对大鼠失血性休克（30毫米汞柱，90分钟）后肠源性内毒素血症/细菌移位以及肿瘤坏死因子（TNF）和白细胞介素-1（IL-1）释放的影响。结果显示，对照组发生了显著的门静脉和全身内毒素血症（门静脉，0.269至0.845内毒素单位（EU）/毫升；全身，0.164至0.655 EU/毫升），而治疗组未发生（门静脉血中0.5小时除外：0.207±0.094 EU/毫升）。同时，接受多粘菌素B的动物在复苏后0.5、2、6和24小时，细菌移位至肠系膜淋巴结和内脏的发生率显著降低（p<0.05至0.01），而两组之间移位细菌的数量没有差异（p>0.05）。在未治疗的对照组中，分别在0.5至2小时（p<0.05）和6至24小时（p<0.05至0.01）也发现血浆TNF水平和腹膜巨噬细胞的IL-1活性显著升高，但低剂量多粘菌素B的给药可预防这种情况。48小时生存率从对照组的41.7%提高到治疗组的75.0%（p>0.05）。这些数据表明，用亚治疗剂量的多粘菌素B预处理可有效抑制出血诱导的肠道内毒素/细菌移位以及TNF和IL-1的过量产生。
