Oka T, Ohwada K, Nagao M, Kitazato K, Kishino Y
Section of Biochemistry, Taiho Pharmaceutical Co Ltd., Tokushima, Japan.
JPEN J Parenter Enteral Nutr. 1994 Nov-Dec;18(6):491-6. doi: 10.1177/0148607194018006491.
Using a transplantable Yoshida sarcoma in a rat model of total parenteral nutrition (TPN), we measured the effectiveness of an arginine-enriched amino acid solution (AI-82) on muscle glutamine concentration and muscle protein synthesis compared with that of a conventional amino acid solution (Proteamin12). After tumor-bearing rats had been given one of two isocaloric TPN regimens for 6 days, [15N]glycine (99 atom %) containing TPN solution was infused into animals at a constant rate of 8 mg of [15N]glycine per hour for 18 hours, after which the liver, skeletal muscle (gastrocnemius muscle), and tumor protein synthesis rates were measured. A significantly increased whole muscle protein synthesis rate was observed in the AI-82 group; there was no difference in the whole liver and tumor protein synthesis rates between the two groups. When each TPN solution was administered for 1 week, muscle concentrations of arginine, ornithine, glutamine, and glutamate were considerably higher in the AI-82 group than in the Proteamin12 group, and these differences were also accompanied by a decrease in the plasma branched-chain amino acid (BCAA) (leucine, isoleucine, and valine) levels in the AI-82 group. The high levels of muscle glutamine concentration in the AI-82 group were investigated in connection with the high use of exogenous branched-chain amino acids.
在全胃肠外营养（TPN）大鼠模型中使用可移植的吉田肉瘤，我们测量了富含精氨酸的氨基酸溶液（AI - 82）与传统氨基酸溶液（Proteamin12）相比，对肌肉谷氨酰胺浓度和肌肉蛋白质合成的有效性。给荷瘤大鼠给予两种等热量TPN方案之一6天后，以每小时8 mg [¹⁵N]甘氨酸的恒定速率向动物输注含[¹⁵N]甘氨酸（99原子%）的TPN溶液18小时，之后测量肝脏、骨骼肌（腓肠肌）和肿瘤的蛋白质合成速率。在AI - 82组中观察到全肌肉蛋白质合成速率显著增加；两组之间全肝脏和肿瘤蛋白质合成速率没有差异。当每种TPN溶液给药1周时，AI - 82组肌肉中的精氨酸、鸟氨酸、谷氨酰胺和谷氨酸浓度明显高于Proteamin12组，并且这些差异还伴随着AI - 82组血浆支链氨基酸（BCAA）（亮氨酸、异亮氨酸和缬氨酸）水平的降低。结合外源性支链氨基酸的高利用率，对AI - 82组中高水平的肌肉谷氨酰胺浓度进行了研究。
