Nakano H, Takamatsu S, Yamamoto Y
Department of Clinico-Laboratory Diagnostics, Nara Medical University.
Nihon Rinsho. 1995 May;53(5):1168-72.
Serum monoamine oxidase (MAO) was separated electrophoretically into three bands. These bands were termed as Fraction I, II and III in that order from anode to cathode. Elevation of serum MAO activity in Fraction I was observed in patients with organ fibrosis particularly in liver cirrhosis and acromegaly. On the other hand, elevation of MAO activity in Fraction II was observed in patients with massive hepatic necrosis. MAO activities partially purified from aortic vessel and from rat liver mitochondria had electrophoretically in the same position as Fraction I and II respectively. Both of Fraction I and aortic extract were inhibited by aminoacetonitrile but not by pargyline. On the contrary, both of Fraction II and mitochondria were inhibited by pargyline but not by aminoacetonitrile. These findings suggested that the elevation of serum MAO in patients with organ fibrosis correlates with the enhancement of connective tissue metabolism and the increment of serum MAO in fulminant hepatitis correlates with the breakaway of MAO from damaged liver mitochondria.
血清单胺氧化酶（MAO）通过电泳分离为三条带。这些带从阳极到阴极依次称为I、II和III组分。在器官纤维化患者中，特别是在肝硬化和肢端肥大症患者中，观察到I组分中血清MAO活性升高。另一方面，在大片肝坏死患者中观察到II组分中MAO活性升高。从主动脉血管和大鼠肝线粒体中部分纯化的MAO活性在电泳中分别与I和II组分处于相同位置。I组分和主动脉提取物均被氨基乙腈抑制，但不被帕吉林抑制。相反，II组分和线粒体均被帕吉林抑制，但不被氨基乙腈抑制。这些发现表明，器官纤维化患者血清MAO升高与结缔组织代谢增强相关，暴发性肝炎患者血清MAO升高与MAO从受损肝线粒体中释放有关。
