Sanguinetti M, Posteraro B, Rossolini G M, Satta G
Istituto di Microbiologia, Università Cattolica del S. Cuore, Rome, Italy.
New Microbiol. 1995 Apr;18(2):117-25.
We have developed a new system suitable for easy inexpensive screening of substances potentially active against HIV and other retroviruses. The system evaluates the ability of substances to inhibit the high-frequency induced retrotransposition of the yeast transposable element Ty917, and is based on an engineered version of the retrotransposon which carries a dominant selectable marker useful for scoring transposition events. The system performance was evaluated using two nucleoside analogues of proved anti-HIV activity, 3'-azido-3'deoxythymidine (AZT) and 2',3'-dideoxycytidine (ddC). Both substances were able to inhibit Ty917 transposition and could have been detected as potentially active antiretroviral drugs using the new screening system.
我们开发了一种新系统，适用于简便且低成本地筛选可能对HIV和其他逆转录病毒具有活性的物质。该系统评估物质抑制酵母转座元件Ty917高频诱导逆转座的能力，并且基于携带用于对转座事件进行评分的显性选择标记的逆转座子工程版本。使用两种已证实具有抗HIV活性的核苷类似物3'-叠氮基-3'-脱氧胸苷（AZT）和2'，3'-双脱氧胞苷（ddC）对系统性能进行了评估。这两种物质都能够抑制Ty917转座，并且使用新的筛选系统本可以被检测为具有潜在活性的抗逆转录病毒药物。
