Gunasegaram R, Peh K L, Chew P C, Karim S M, Ratnam S S
Acta Endocrinol (Copenh). 1979 Jan;90(1):185-92. doi: 10.1530/acta.0.0900185.
Homogenates of first trimester human placentae were incubated with [26-14C]chloresterol. Using reverse-isotope dilution analysis [14C]isocaproic acid was isolated and characterized. A progressive increase in [14C]isocaproic acid formation with increasing gestation suggestive of a regulatory mechanism at the C-20, 22 desmolase site in the supply of placental pregnenolone was noted.
将孕早期人胎盘匀浆与[26-¹⁴C]胆固醇一起孵育。采用反向同位素稀释分析法分离并鉴定了[¹⁴C]异己酸。结果发现，随着妊娠进展，[¹⁴C]异己酸的生成逐渐增加，这表明在胎盘孕烯醇酮供应过程中，C-20、22碳链裂解酶位点存在调节机制。
