Oohira A, Kushima Y, Matsui F, Watanabe E
Department of Perinatology and Neuroglycoscience, Institute for Developmental Research, Aichi, Japan.
Neurosci Lett. 1995 Apr 7;189(1):25-8. doi: 10.1016/0304-3940(95)11441-x.
Alzheimer's beta-amyloid precursor related proteins bearing chondroitin sulfate chains were detected in the conditioned media of primary cultured astrocytes obtained from fetal rat brains by Western blotting using the monoclonal antibody 22C11 against Alzheimer's beta-amyloid precursor protein (APP), but not in the media of cortical neurons. The chondroitin sulfate proteoglycan form of APP was also detectable in a soluble proteoglycan fraction prepared from 10-day-old rat brains. However, the amount of proteoglycan form of APP in the brain was very small compared to non-proteoglycan forms at all the developmental stages from embryonic day 14 to 2 years. These observations suggest that astrocytes are one cellular source of the proteoglycan form of APP in the brain.
利用抗阿尔茨海默病β淀粉样前体蛋白（APP）的单克隆抗体22C11，通过蛋白质印迹法在从胎鼠脑获得的原代培养星形胶质细胞的条件培养基中检测到带有硫酸软骨素链的阿尔茨海默病β淀粉样前体相关蛋白，但在皮质神经元的培养基中未检测到。在从10日龄大鼠脑制备的可溶性蛋白聚糖组分中也可检测到APP的硫酸软骨素蛋白聚糖形式。然而，从胚胎第14天到2岁的所有发育阶段，脑中APP的蛋白聚糖形式的量与非蛋白聚糖形式相比非常少。这些观察结果表明星形胶质细胞是脑中APP蛋白聚糖形式的一个细胞来源。
