Sung M K, Kendall C W, Koo M M, Rao A V
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Ontario, Canada.
Nutr Cancer. 1995;23(3):259-70. doi: 10.1080/01635589509514380.
The effects of soybean saponins (SS) and gypsophilla saponin (GS) on the growth and viability of colon tumor (HCT-15) cells in culture were investigated. Cells were incubated in various concentrations of saponins for 1 hour (short term) or 48 hours (long term). Cell growth and viability were monitored at 24 and 48 hours. SS and GS inhibited cell growth and reduced cell viability in a dose-dependent manner in long-term treatment. The viability of cells was also reduced by short-term treatment with GS. The saponins differed in their effects on cell surface morphology: GS induced a rough and granular cell surface, whereas SS-treated cells displayed only minor morphological alterations. Changes in membrane permeability were assessed by measuring leakage of the cytoplasmic enzyme lactate dehydrogenase from cells. GS showed a concentration-dependent increase in lactate dehydrogenase leakage, whereas SS did not exhibit this effect. These results suggest that SS and GS have a significant growth-inhibitory effect on colon tumor cells in culture. However, it would appear that they are acting through different mechanisms.
研究了大豆皂苷（SS）和桔梗皂苷（GS）对培养的结肠肿瘤（HCT - 15）细胞生长和活力的影响。将细胞在不同浓度的皂苷中孵育1小时（短期）或48小时（长期）。在24小时和48小时监测细胞生长和活力。长期处理时，SS和GS以剂量依赖的方式抑制细胞生长并降低细胞活力。短期用GS处理也降低了细胞活力。皂苷对细胞表面形态的影响不同：GS诱导细胞表面粗糙且呈颗粒状，而经SS处理的细胞仅表现出轻微的形态改变。通过测量细胞质酶乳酸脱氢酶从细胞中的泄漏来评估膜通透性的变化。GS显示乳酸脱氢酶泄漏呈浓度依赖性增加，而SS未表现出这种效应。这些结果表明，SS和GS对培养的结肠肿瘤细胞具有显著的生长抑制作用。然而，它们似乎是通过不同的机制起作用。
