Lando G, Asonganyi T, Ngogang J, Ngu J L
University Centre for Health Sciences, University of Yaounde, Cameroon.
Afr J Med Med Sci. 1994 Sep;23(3):261-7.
Lipid extraction of adult O. volvulus worms using a chloroform/methanol/water mixture yielded 10 lipid fractions of which 8 were demonstrated by the orcinol reagent to be glycolipids. In TLC, two of these lipid fractions had mobilities similar to cholesterol and cholesterol ester (Rf.: 0.95, 0.86) whereas two others migrated as sphingomyelin and lecithin (Rf.: 0.40, 0.35) respectively. Other components migrated at intermediate positions. The glycolipids were immunologically active and reacted with sera from onchocerciasis patients. The highest reaction was obtained with the IgG antibody class, followed by IgM while no appreciable reactivity was observed with IgE. Sera from patients infected with other filariae such as Loa-loa and Dipetalonema perstans did not show any significant reaction with these antigens. The significance of these results is discussed.
使用氯仿/甲醇/水混合物对成年旋盘尾丝虫进行脂质提取，得到了10个脂质组分，其中8个经地衣酚试剂鉴定为糖脂。在薄层色谱法中，这些脂质组分中有两个的迁移率与胆固醇和胆固醇酯相似（比移值：0.95、0.86），而另外两个分别以鞘磷脂和卵磷脂的形式迁移（比移值：0.40、0.35）。其他组分在中间位置迁移。这些糖脂具有免疫活性，能与盘尾丝虫病患者的血清发生反应。与IgG抗体类别反应最强，其次是IgM，而与IgE未观察到明显反应。感染其他丝虫如罗阿罗阿丝虫和常现棘唇线虫的患者血清与这些抗原未显示任何显著反应。讨论了这些结果的意义。
