Zhao B, Rickert C H, Filler T J, Liu B, Verhallen P F, Dierichs R
Institute of Anatomy, University of Münster, Germany.
Am J Hematol. 1995 Jul;49(3):177-82. doi: 10.1002/ajh.2830490302.
In order to study the influence of oxidized low-density lipoprotein (Ox-LDL) on platelet functional morphology at an early activation stage, washed human blood platelets were stimulated by 100 micrograms/ml Ox-LDL at 37 degrees C. The settling and spreading process of stimulated and unstimulated platelets on Formvar-coated glass was observed for approximately 20 min by reflection contrast microscopy (RCM) and quantified by image analysis. Each group consisted of at least 250 platelets. The results show that incubation with Ox-LDL causes platelet shape change and pseudopodia formation. The sedimentation of stimulated platelets precedes that of unstimulated platelets by approximately 3 min. The increase of the total adhesion area of all Ox-LDL treated platelets is significantly accelerated in comparison to normal platelets (20.45 microns2/min vs. 15.45 microns2/min; P < 0.01). The mean total adhesion area of Ox-LDL-treated platelets was generally larger than that of untreated platelets (189.7 microns2 vs. 144.7 microns2; P < 0.01). The disappearance of intracellular granules after platelet activation, observed by RCM, is supported by transmission electron microscopy. Our results suggest that Ox-LDL activates platelets and advances and accelerates their adhesion and thereby may contribute to pathological thrombosis and arteriosclerosis.
为了研究氧化型低密度脂蛋白（Ox-LDL）在早期激活阶段对血小板功能形态的影响，将洗涤后的人血血小板在37℃下用100微克/毫升的Ox-LDL进行刺激。通过反射对比显微镜（RCM）观察刺激和未刺激的血小板在福尔马肼包被玻片上的沉降和铺展过程约20分钟，并通过图像分析进行量化。每组至少包含250个血小板。结果显示，与Ox-LDL孵育会导致血小板形状改变和伪足形成。刺激后的血小板沉降比未刺激的血小板提前约3分钟。与正常血小板相比，所有经Ox-LDL处理的血小板的总黏附面积增加明显加快（20.45平方微米/分钟对15.45平方微米/分钟；P<0.01）。经Ox-LDL处理的血小板的平均总黏附面积通常大于未处理的血小板（189.7平方微米对144.7平方微米；P<0.01）。通过RCM观察到的血小板激活后细胞内颗粒的消失得到了透射电子显微镜的支持。我们的结果表明，Ox-LDL激活血小板并促进和加速其黏附，从而可能导致病理性血栓形成和动脉粥样硬化。
