Cifuentes-Diaz C, Nicolet M, Alameddine H, Goudou D, Dehaupas M, Rieger F, Mège R M
INSERM U. 153, CNRS ERS 64, Paris, France.
Mech Dev. 1995 Mar;50(1):85-97. doi: 10.1016/0925-4773(94)00327-j.
In this work, we investigated the distribution of the Ca(2+)-dependent cell adhesion molecule, M-cadherin, in mouse limb muscle during normal development and regeneration. Using two unrelated anti-M-cadherin peptide antibodies, we found scarce M-cadherin immunostaining during primary myogenesis (E12-E14) with no accumulation at areas of cell-cell contact. In contrast, the staining sharply increased in intensity at E16, remained high during secondary myogenesis (E16-P0) but disappeared soon after birth. During secondary myogenesis, M-cadherin was specifically accumulated at the characteristic sites of insertion of secondary myotubes in neighbouring primary myotubes. M-cadherin was also accumulated at the areas of contact between fusing secondary myoblasts and myotubes in vitro. In the adult normal and regenerating muscle, we did not detect M-cadherin accumulations at the surface of myofibres. All together, these observations suggest that M-cadherin is specifically involved in secondary myogenesis.
在本研究中，我们调查了Ca(2+)依赖的细胞粘附分子M-钙粘蛋白在小鼠肢体肌肉正常发育和再生过程中的分布情况。使用两种不相关的抗M-钙粘蛋白肽抗体，我们发现在原发性肌生成（E12-E14）期间M-钙粘蛋白免疫染色稀少，在细胞间接触区域无积累。相比之下，在E16时染色强度急剧增加，在继发性肌生成（E16-P0）期间保持高水平，但在出生后不久消失。在继发性肌生成过程中，M-钙粘蛋白特异性积累在继发性肌管插入相邻原发性肌管的特征部位。M-钙粘蛋白也在体外融合的继发性成肌细胞与肌管之间的接触区域积累。在成年正常和再生肌肉中，我们未在肌纤维表面检测到M-钙粘蛋白积累。总之，这些观察结果表明M-钙粘蛋白特别参与继发性肌生成。
