肌钙蛋白I和T在实验性心肌肥大中的蛋白表达

Troponin I and T protein expression in experimental cardiac hypertrophy.

作者信息

Cumming D V, Seymour A M, Rix L K, Kellett R, Dhoot G K, Yacoub M H, Barton P J

机构信息

Department of Cardiothoracic Surgery, National Heart and Lung Institute, London.

出版信息

Cardioscience. 1995 Mar;6(1):65-70.

PMID:7605898

Abstract

We have examined four models of experimental cardiac hypertrophy and heart failure for alterations in troponin isoform expression, particularly in the re-expression of the fetal isoforms. Cardiac protein extracts from experimental and sham-operated control rats were analyzed using one dimensional gel electrophoresis, followed by Western blotting and detection with antibodies specific for troponin I and T. No alteration in protein profile was observed for these proteins between control, hypertrophied and failing heart samples. The data demonstrate that reversion to the fetal pattern of troponin expression is not a feature of experimental cardiac hypertrophy and heart failure in the rat.

摘要

我们研究了四种实验性心脏肥大和心力衰竭模型中肌钙蛋白异构体表达的变化，特别是胎儿异构体的重新表达情况。使用一维凝胶电泳对实验大鼠和假手术对照大鼠的心脏蛋白提取物进行分析，随后进行蛋白质印迹分析，并用针对肌钙蛋白I和T的特异性抗体进行检测。在对照、肥大和衰竭心脏样本之间，未观察到这些蛋白质的蛋白质谱有任何变化。数据表明，肌钙蛋白表达恢复到胎儿模式并非大鼠实验性心脏肥大和心力衰竭的特征。