Premkumar L, Chung S H
Department of Chemistry, Australian National University, Canberra.
Neuroreport. 1995 Mar 27;6(5):765-8. doi: 10.1097/00001756-199503270-00015.
Single channel potassium currents activated indirectly via stimulation of metabotropic receptors were recorded in cell-attached patches on hippocampal neurones in slices from neonatal rats. When metabotropic receptors in the area outside of the patch are stimulated by glutamate, quisqualate and trans-ACPD, single channel currents appear with a predominant conductance level of about 20 pS. From the reversal potential and substitution of ionic species, we established that the currents are carried by potassium ions. Using selective blockers of ionotropic receptors in conjunction with specific agonists for the metabotropic receptor, it was ascertained that the channel observed is linked to metabotropic receptors. The currents display submaximal conductance states and fluctuate rapidly between evenly-spaced conductance sublevels.
在新生大鼠脑片海马神经元的细胞贴附式膜片上，记录到通过代谢型受体刺激间接激活的单通道钾电流。当在膜片外区域的代谢型受体受到谷氨酸、quisqualate和反式-ACPD刺激时，会出现单通道电流，其主要电导水平约为20 pS。根据反转电位和离子种类的替代，我们确定这些电流是由钾离子携带的。使用离子型受体的选择性阻断剂与代谢型受体的特异性激动剂相结合，确定观察到的通道与代谢型受体相关联。这些电流呈现亚最大电导状态，并在均匀间隔的电导亚水平之间快速波动。
