Soravia E, Grebe A, De Luca P, Helin K, Suh T T, Degen J L, Blasi F
Department of Genetics and Microbiology, University of Milano, Italy.
Blood. 1995 Jul 15;86(2):624-35.
The urokinase-type plasminogen activator receptor (uPAR) focuses at the cell surface the activation of pro-uPA and, hence, the formation of plasmin, thus enhancing directional extracellular proteolysis. To characterize the transcriptional regulatory mechanisms that control receptor expression, we have cloned an uPAR DNA segment containing upstream regulatory sequences from both the human and murine genomes. We report that a proximal promoter, contained within 180 bp from the major transcription start sites of the human uPAR gene, drives basal transcription. This region lacks TATA and CAAT boxes and contains relatively GC-rich proximal sequences. A subregion of this sequence, highly conserved between human and murine genes, contains most of the promoter activity and is specifically bound by HeLa nuclear proteins, one of which belongs to the SP1 class.
尿激酶型纤溶酶原激活物受体（uPAR）在细胞表面使单链尿激酶型纤溶酶原激活物（pro-uPA）活化，进而形成纤溶酶，从而增强定向细胞外蛋白水解作用。为了阐明调控该受体表达的转录调节机制，我们从人类和小鼠基因组中克隆了一段包含上游调控序列的uPAR DNA片段。我们发现，人uPAR基因主要转录起始位点上游180 bp范围内的近端启动子可驱动基础转录。该区域缺乏TATA盒和CAAT盒，含有相对富含GC的近端序列。此序列中的一个亚区域在人类和小鼠基因之间高度保守，包含大部分启动子活性，并且能与HeLa细胞核蛋白特异性结合，其中一种核蛋白属于SP1家族。
