Highsmith F, Xue H, Chen X, Benade L, Owens J, Shanbrom E, Drohan W
Holland Laboratory, Plasma Derivatives Department, American Red Cross, Rockville, MD 20855, USA.
Blood. 1995 Jul 15;86(2):791-6.
Human plasma-derived protein concentrates intended for clinical use must be treated for viral inactivation to ensure patient safety. This study explored the use of liquid iodine for inactivation of several lipid- and nonlipid-enveloped viruses in an antithrombin III (AT-III) concentrate. Iodine at levels of 0.01% to 0.02% caused between 43% and 94% loss of AT-III activity, as well as degradation of AT-III as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. However, addition of up to 0.1% human albumin protected the AT-III against both inactivation and fragmentation. At albumin levels sufficient to retain greater than 75% of AT-III activity, greater than 6 logs of sindbis, encephalomyocarditis, and vesicular stomatitis viruses, greater than 4 logs of pseudorabies, and greater than 3 logs of human immunodeficiency virus were inactivated. Except with sindbis virus, this represented complete inactivation of all the viruses spiked into the AT-III concentrate.
用于临床的人血浆源性蛋白浓缩物必须进行病毒灭活处理，以确保患者安全。本研究探讨了使用液态碘对抗凝血酶III（AT-III）浓缩物中的几种有包膜和无包膜病毒进行灭活。0.01%至0.02%的碘会导致AT-III活性损失43%至94%，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质免疫印迹分析显示AT-III也会发生降解。然而，添加高达0.1%的人白蛋白可保护AT-III不被灭活和裂解。在白蛋白水平足以保留大于75%的AT-III活性时，辛德毕斯病毒、脑心肌炎病毒和水疱性口炎病毒的灭活率大于6个对数，伪狂犬病病毒的灭活率大于4个对数，人类免疫缺陷病毒的灭活率大于3个对数。除辛德毕斯病毒外，这代表了加入到AT-III浓缩物中的所有病毒均被完全灭活。
