Batista U, Sentjurc M
Institute of Biophysics, Medical Faculty, University of Ljubljana, Slovenia.
Cell Biol Int. 1995 Mar;19(3):215-22. doi: 10.1006/cbir.1995.1064.
Electron paramagnetic resonance (EPR) was used to study the effect of equinatoxin II (EqT II), a cytolytic protein isolated from the sea anemone Actinia equina L., on membrane fluidity and cell metabolism of V-79 cells; the reduction of the spin probe incorporated into the cell membranes as well as the oxygen consumption in the cell suspension were measured. The results were compared with the results obtained by the cell viability study. Under the influence of EqT II (less than 37.5 micrograms/10(6) cells) no significant changes in cell membrane fluidity were observed, while reduction kinetics of the spin probe and the oxygen consumption decreased when the cells were kept in Tris buffer solution. However, in the presence of 10% fetal calf serum, which prevented cell lysis, the effects of EqT II were diminished. The oxygen consumption corresponds to the cell viability changes but the reduction kinetics alterations indicate that some oxidation-reduction processes other than cell respiration are affected by EqT II in the absence of serum. The effect seems to be indirect, probably due to the formation of pores which are associated with changed permeability of plasmalemma for metabolites and ions.
利用电子顺磁共振（EPR）研究了从海葵平展列指海葵中分离出的细胞溶解蛋白海葵毒素II（EqT II）对V - 79细胞的膜流动性和细胞代谢的影响；测量了掺入细胞膜中的自旋探针的减少以及细胞悬液中的耗氧量。将结果与细胞活力研究所得结果进行了比较。在EqT II（小于37.5微克/10⁶个细胞）的影响下，未观察到细胞膜流动性有显著变化，而当细胞置于Tris缓冲溶液中时，自旋探针的减少动力学和耗氧量降低。然而，在存在10%胎牛血清（可防止细胞裂解）的情况下，EqT II的作用减弱。耗氧量与细胞活力变化相对应，但减少动力学的改变表明，在无血清时，除细胞呼吸外的一些氧化还原过程受到EqT II的影响。这种作用似乎是间接的，可能是由于形成了与质膜对代谢物和离子通透性改变相关的孔。
