Illanes A, Torres R, Cartagena O, Ruiz A
Escuela de Ingeniería Bioquímica, Universidad Católica de Valparaíso, Chile.
Biol Res. 1993;26(3):357-64.
Penicillin acylase is a key enzyme for the production of semisynthetic beta-lactam antibiotics. The intracellular enzyme from Escherichia coli has been thoroughly studied and characterized. The extracellular enzyme from Bacillus megaterium, despite its potential advantages, has received less attention in the recent scientific literature. A comparative study is presented for the production of penicillin acylase with two strains of Bacillus megaterium in batch fermentation in previously optimized complex and defined media. The enzyme produced by the selected strain has been recovered, partially purified and its kinetic behaviour determined.
青霉素酰化酶是生产半合成β-内酰胺抗生素的关键酶。来自大肠杆菌的胞内酶已得到充分研究和表征。巨大芽孢杆菌的胞外酶尽管具有潜在优势，但在最近的科学文献中受到的关注较少。本文针对在先前优化的复合培养基和限定培养基中利用两株巨大芽孢杆菌进行分批发酵生产青霉素酰化酶开展了一项比较研究。所选菌株产生的酶已被回收、部分纯化并测定了其动力学行为。
