Bode-Böger S M, Böger R H, Schröder E P, Frölich J C
Institute of Clinical Pharmacology, Hanover Medical School, Germany.
J Cardiovasc Risk. 1994 Aug;1(2):173-8.
Our aim was to compare the effects of exercise on systemic nitric oxide formation, assessed from the urinary excretion rates of nitrate and of cyclic GMP, in endurance-trained and untrained men.
Ten endurance-trained and six untrained men underwent a submaximal exercise test for 30 min at 60% of their individual maximal work capacity. Urinary excretion rates of nitrate (measured using gas chromatography) and cyclic GMP (measured using a radioimmunoassay) were assessed at hourly intervals.
Urinary nitrate excretion was comparable at rest in the two groups (104 +/- 35 and 110 +/- 19 mumol/mmol creatinine; NS), more than doubled during exercise in both groups (236 +/- 88 mumol/mmol creatinine in untrained and 252 +/- 39 mumol/mmol creatinine in trained participants; P < 0.01 for both groups) and decreased rapidly after the completion of the exercise test. Excretion of urinary cyclic GMP at rest was four times higher in the endurance-trained than in the untrained men (21 +/- 5 compared with 6 +/-1 nmol/mmol creatinine; P < 0.05). During the exercise test, GMP excretion approximately doubled in both groups (52 +/- 12 and 10 +/- 1 nmol/mmol creatinine, respectively; P < or = 0.01 for both groups) and returned to baseline at the end of the test. Urinary cyclic GMP and nitrate excretion correlated well in both groups (p < 0.05). Atrial natriuretic factor is also a stimulator of cyclic GMP formation but resting plasma levels of atrial natriuretic factor did not differ between the groups (22 +/- 3 compared with 21 +/- 4 pmol/l; NS).
An acute submaximal exercise test increases the formation of nitric oxide, reflected in the increase in urinary excretion of nitrate and cyclic GMP. This increase may contribute to vasodilatation during physical exercise and at least partly explain the beneficial effects of physical training in patients with vascular diseases.
我们的目的是比较运动对耐力训练和未训练男性体内一氧化氮生成的影响，一氧化氮生成通过硝酸盐和环磷酸鸟苷的尿排泄率来评估。
10名耐力训练男性和6名未训练男性以个人最大工作能力的60%进行30分钟的次极量运动测试。每小时评估一次硝酸盐（采用气相色谱法测量）和环磷酸鸟苷（采用放射免疫分析法测量）的尿排泄率。
两组在静息时尿硝酸盐排泄相当（分别为104±35和110±19μmol/mmol肌酐；无显著性差异），两组在运动期间均增加了一倍多（未训练者为236±88μmol/mmol肌酐，训练者为252±39μmol/mmol肌酐；两组P均<0.01），运动测试结束后迅速下降。耐力训练男性静息时尿环磷酸鸟苷排泄量是未训练男性的四倍（分别为21±5和6±1nmol/mmol肌酐；P<0.05）。在运动测试期间，两组环磷酸鸟苷排泄量均增加了约一倍（分别为52±12和10±1nmol/mmol肌酐；两组P≤0.01），测试结束时恢复到基线水平。两组尿环磷酸鸟苷和硝酸盐排泄量相关性良好（p<0.05）。心钠素也是环磷酸鸟苷生成的刺激物，但两组静息时血浆心钠素水平无差异（分别为22±3和21±4pmol/L；无显著性差异）。
一次急性次极量运动测试增加了一氧化氮的生成，这反映在尿硝酸盐和环磷酸鸟苷排泄量的增加上。这种增加可能有助于体育锻炼期间的血管舒张，并至少部分解释体育锻炼对血管疾病患者的有益作用。
