Brzoska P M, Levin N A, Fu K K, Kaplan M J, Singer M I, Gray J W, Christman M F
Department of Radiation Oncology, University of California, San Francisco 94143, USA.
Cancer Res. 1995 Jul 15;55(14):3055-9.
We have undertaken a study of DNA copy number changes in head and neck squamous cell carcinomas to identify novel DNA copy number changes and to determine the significance of previous findings of cytogenetic alterations in cultured cells. Comparative genomic hybridization was performed on genomic DNA extracted from ten tumors. A novel copy number gain on chromosome 3q26-27 and a loss of chromosome 3p were found at high frequency (> or = 50% of tumors). Many other novel chromosomal copy number changes were identified but occurred at a lower frequency. In addition, our data confirm that DNA copy number changes that frequently occur in cultured cells, such as loss of chromosome 3p, also occur in tumors. Frequently altered loci may encode oncogenes or tumor suppressor genes involved in head and neck squamous cell carcinoma tumorigenesis.
我们开展了一项关于头颈部鳞状细胞癌DNA拷贝数变化的研究，以识别新的DNA拷贝数变化，并确定之前在培养细胞中细胞遗传学改变研究结果的意义。对从10个肿瘤中提取的基因组DNA进行了比较基因组杂交。在3号染色体q26 - 27区域发现了新的拷贝数增加，3号染色体p臂缺失在肿瘤中高频出现（≥50%的肿瘤）。还识别出许多其他新的染色体拷贝数变化，但出现频率较低。此外，我们的数据证实，在培养细胞中经常出现的DNA拷贝数变化，如3号染色体p臂缺失，在肿瘤中也会出现。频繁改变的基因座可能编码参与头颈部鳞状细胞癌肿瘤发生的癌基因或肿瘤抑制基因。
