Fernandez de Henestrosa A R, Rivera E, Barbé J
Department of Genetics and Microbiology, Faculty of Sciences, Autonomous University of Barcelona, Bellaterra, Spain.
FEMS Microbiol Lett. 1995 Jun 15;129(2-3):175-81. doi: 10.1111/j.1574-6968.1995.tb07576.x.
The Rhodobacter capsulatus recA gene has been isolated and sequenced. Its deduced amino acid sequence showed the closest identity with the Rhodobacter sphaeroides RecA protein (91% identity). However, the promoter regions of both R. capsulatus and R. sphaeroides recA genes are only 64% similar. An Escherichia coli-like LexA binding site was not present in the upstream region of the R. capsulatus recA gene. Nevertheless, the R. capsulatus recA gene is inducible by DNA damage in both hetero- and phototrophically growing conditions. The R. capsulatus recA gene is poorly induced when inserted into the chromosome of R. sphaeroides, indicating that the recA gene of both bacteria possess different control sequences despite their phylogenetically close relationship.
荚膜红杆菌recA基因已被分离并测序。其推导的氨基酸序列与球形红杆菌RecA蛋白的一致性最高（91%一致性）。然而，荚膜红杆菌和球形红杆菌recA基因的启动子区域仅64%相似。荚膜红杆菌recA基因的上游区域不存在类似大肠杆菌的LexA结合位点。尽管如此，荚膜红杆菌recA基因在异养和光合营养生长条件下均能被DNA损伤诱导。当将荚膜红杆菌recA基因插入球形红杆菌染色体时，其诱导效果较差，这表明尽管两种细菌在系统发育上关系密切，但它们的recA基因拥有不同的调控序列。
