Timinskas A, Butkus V, Janulaitis A
Institute of Biotechnology FERMENTAS, Vilnius, Lithuania.
Gene. 1995 May 19;157(1-2):3-11. doi: 10.1016/0378-1119(94)00783-o.
Two additional conserved motifs (CM), CM Is and CM III, have been found in addition to well-known CM I and CM II within the primary amino acid sequences of almost all m6A- and m4C-methyltransferases (MTases). The boundaries of all four CM were defined and their consensus sequences characteristic both for different classes, as well as for all N-MTases, were derived. Some regular deviations at fixed positions of the consensus sequences CM Is, CM I and CM II, typical for separate classes of N-MTases, were presumed to correlate. A possible structural basis for the supposed interregional correlations is discussed and experiments for verification of the assumed interactions between CM are suggested. A classification scheme for all N-MTases is provided.
除了几乎所有m6A和m4C甲基转移酶（MTases）一级氨基酸序列中已知的保守基序I（CM I）和保守基序II（CM II）之外，还发现了另外两个保守基序，即保守基序Is（CM Is）和保守基序III（CM III）。确定了所有四个保守基序的边界，并推导了它们对于不同类别以及所有N-甲基转移酶而言的共有序列特征。推测保守基序Is、CM I和CM II共有序列固定位置上的一些典型的、特定于不同类别的N-甲基转移酶的规则偏差存在相关性。讨论了假定的区域间相关性的可能结构基础，并提出了验证保守基序之间假定相互作用的实验。提供了所有N-甲基转移酶的分类方案。
