Mason P J, Stevens D J, Luzzatto L, Brenner S, Aparicio S
Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
Genomics. 1995 Apr 10;26(3):587-91. doi: 10.1016/0888-7543(95)80179-p.
To investigate the organization of the compact genome of the puffer fish Fugu rubripes at the level of an individual housekeeping gene, we have cloned and sequenced the glucose-6-phosphate dehydrogenase (G6PD) gene from this fish and compared it to the corresponding human gene. The intron/exon structure of the two genes is identical throughout the protein coding regions. The puffer fish gene is four times smaller than the human gene--the difference is accounted for by the fact that the puffer fish gene has smaller introns. Intron 2 is the largest intron in both species. We have constructed a molecular phylogeny for 10 G6PD protein sequences. The sequences fall in the expected arrangement based on established phylogenetic relationships, with the Plasmodium falciparum sequence the most widely diverged.
为了在单个管家基因水平上研究河豚红鳍东方鲀紧凑基因组的组织情况，我们克隆并测序了该鱼的葡萄糖-6-磷酸脱氢酶（G6PD）基因，并将其与相应的人类基因进行了比较。这两个基因的内含子/外显子结构在整个蛋白质编码区域都是相同的。河豚基因比人类基因小四倍——差异的原因是河豚基因的内含子较小。内含子2是两个物种中最大的内含子。我们构建了10个G6PD蛋白质序列的分子系统发育树。根据已建立的系统发育关系，这些序列呈预期排列，恶性疟原虫序列分歧最大。
