Tagaya M, Toyonaga S, Takahashi M, Yamamoto A, Fujiwara T, Akagawa K, Moriyama Y, Mizushima S
School of Life Science, Tokyo University of Pharmacy and Life Science, Japan.
J Biol Chem. 1995 Jul 7;270(27):15930-3. doi: 10.1074/jbc.270.27.15930.
Syntaxin 1 (HPC-1), a component of the receptor for SNAPs (soluble N-ethylmaleimide-sensitive factor attachment proteins), has been implicated in the docking and fusion of synaptic vesicles with the plasma membrane. It was reported that syntaxin 1 in rat brain and chromaffin cells (PC12) is exclusively located on the plasma membrane (Bennett, M. K., Calakos, N., and Scheller, R. H. (1992) Science 257, 255-259; Söllner, T., Bennett, M. K., Whiteheart, S. W., Scheller, R. H., and Rothman, J. E. (1993) Cell 75, 409-418). By means of biochemical and morphological analyses, we now show that syntaxin 1 is associated with chromaffin granules in the adrenal medulla. This finding raises the possibility that syntaxin 1 in chromaffin cells is a component of vesicle-SNAP receptor as well as one of target-SNAP receptor on the plasma membrane.
Syntaxin 1（HPC-1）是可溶性N-乙基马来酰亚胺敏感因子附着蛋白（SNAPs）受体的一个组成部分，与突触小泡和质膜的对接及融合有关。据报道，大鼠脑和嗜铬细胞（PC12）中的Syntaxin 1仅位于质膜上（贝内特，M.K.，卡拉科斯，N.，和谢勒，R.H.（1992年）《科学》257卷，255 - 259页；索尔纳，T.，贝内特，M.K.，怀特哈特，S.W.，谢勒，R.H.，和罗斯曼，J.E.（1993年）《细胞》75卷，409 - 418页）。通过生化和形态学分析，我们现在表明Syntaxin 1与肾上腺髓质中的嗜铬颗粒相关。这一发现增加了一种可能性，即嗜铬细胞中的Syntaxin 1是囊泡-SNAP受体的一个组成部分，也是质膜上靶标-SNAP受体之一。
