Tagaya M, Genma T, Yamamoto A, Kozaki S, Mizushima S
School of Life Science, Tokyo University of Pharmacy and Life Science, Japan.
FEBS Lett. 1996 Sep 23;394(1):83-6. doi: 10.1016/0014-5793(96)00932-5.
SNAP-25 is located on the plasma membrane and essential for exocytosis of neurotransmitters. It was suggested that SNAP-25 and syntaxin 1 via the interaction with VAMP-2 located on synaptic vesicles mediate the docking of the vesicles with the plasma membrane. In the present study, by means of biochemical and morphological analyses, we showed that SNAP-25 is present on chromaffin granules as well as on the plasma membrane. Reconstitution and immunoprecipitation analyses revealed that SNAP-25 on chromaffin granules has essentially the same properties as does SNAP-25 on the plasma membrane.
突触小体相关蛋白25（SNAP - 25）位于质膜上，对神经递质的胞吐作用至关重要。有研究表明，SNAP - 25和 syntaxin 1通过与位于突触小泡上的囊泡相关膜蛋白2（VAMP - 2）相互作用，介导小泡与质膜的对接。在本研究中，通过生化和形态学分析，我们发现SNAP - 25不仅存在于质膜上，也存在于嗜铬颗粒上。重组和免疫沉淀分析表明，嗜铬颗粒上的SNAP - 25与质膜上的SNAP - 25基本具有相同的特性。
