Henkel C K, Brunso-Bechtold J K
Department of Neurobiology and Anatomy, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27157-1010, USA.
J Comp Neurol. 1995 Apr 10;354(3):470-80. doi: 10.1002/cne.903540313.
The distribution of glycine-immunopositive cells and axonal endings was studied in the adult and early postnatal ferret superior olive. As in other species, the most prominent glycine-immunopositive cell group in the adult ferret superior olive was the medial nucleus of the trapezoid body. Other darkly immunostained cells were present, although more scattered, in most periolivary regions, including the lateral and ventral trapezoid body nuclei. In the lateral superior olivary nuclei, glycine-immunopositive cells were intermingled with immunonegative cells. A comparable population of cells in the ipsilateral lateral superior olivary nucleus was retrogradely labeled in cases with unilateral injections of tritiated glycine in the inferior colliculus. Glycine-immunopositive puncta were widely distributed in the neuropil in most periolivary regions, including dense accumulations in the dorsomedial periolivary region and ventral and lateral nuclei of the trapezoid body. In the lateral and medial superior olivary nuclei, immunopositive puncta were distributed around the principal cells in characteristic perisomatic halos. In postnatal ferrets, immunopositive cell bodies were first observed by postnatal day 7 and were distributed in regions comparable to regions in the adult, with the exception that immunopositive cells in the lateral superior olivary nucleus did not appear until about postnatal day 28. There was diffuse staining in the neuropil in principal and periolivary nuclei by postnatal day 7. During the third postnatal week, the immunostaining in the neuropil began to take on a more granular appearance and immunopositive puncta could be seen by postnatal day 35. In the lateral and medial superior olivary nuclei, the earliest distribution of immunostaining in the neuropil was nonuniform, being greater in the high-frequency, medial, and ventral regions, respectively. The density gradient in these areas was gradually eliminated over the next 2 postnatal weeks as immunostained processes and endings appeared over greater portions of the nuclei.
研究了成年和出生后早期雪貂上橄榄核中甘氨酸免疫阳性细胞和轴突终末的分布。与其他物种一样，成年雪貂上橄榄核中最突出的甘氨酸免疫阳性细胞群是梯形体内侧核。在大多数橄榄周区域，包括外侧和腹侧梯形体核，也存在其他免疫染色较深的细胞，不过分布更为分散。在外侧上橄榄核中，甘氨酸免疫阳性细胞与免疫阴性细胞相互混杂。在下丘单侧注射氚标记甘氨酸的情况下，同侧外侧上橄榄核中有相当数量的细胞被逆行标记。甘氨酸免疫阳性斑点广泛分布于大多数橄榄周区域的神经毡中，包括背内侧橄榄周区域以及梯形体腹侧和外侧核中的密集聚集区。在外侧和内侧上橄榄核中，免疫阳性斑点以特征性的胞体周围光环形式分布在主细胞周围。在出生后的雪貂中，免疫阳性细胞体最早在出生后第7天被观察到，其分布区域与成年雪貂的区域相当，不同之处在于外侧上橄榄核中的免疫阳性细胞直到出生后约第28天才出现。到出生后第7天，主核和橄榄周核的神经毡中出现弥漫性染色。在出生后第三周，神经毡中的免疫染色开始呈现出更颗粒状的外观，到出生后第35天可以看到免疫阳性斑点。在外侧和内侧上橄榄核中，神经毡中免疫染色的最早分布是不均匀的，分别在高频、内侧和腹侧区域更为明显。在接下来的2周出生后时间里，随着免疫染色的突起和终末出现在核的更大区域，这些区域的密度梯度逐渐消失。
