Otsuki T, Oku M
Department of Obstetrics and Gynecology, Nara Medical University.
Nihon Sanka Fujinka Gakkai Zasshi. 1995 Jun;47(6):531-8.
Glucocorticoids inhibit prostaglandin synthesis in several cell types, presumably by inhibiting arachidonic acid deacylation from phospholipids. We studied the effects of cortisol (F) and progesterone (P) on fatty acid release from cultured human myometrial cells. Confluent monolayer cultures of myometrial cells were adapted to steroids containing medium for 24 hours and the intracellular arachidonic acid and palmitic acid concentrations were determined. In the presence of fetal calf serum (FCS), the palmitic acid concentrations significantly decreased after the addition of 10(-7)M F. In the absence of FCS, the concentrations of both fatty acids were markedly increased after the addition of F. During oxytocin stimulation, the arachidonic acid concentration did not change but the palmitic acid concentration decreased slightly after the addition of F. A similar evaluation was done with P. The palmitic acid concentration decreased slightly after the addition of 10(-5)M P in the presence of FCS but increased markedly in the absence of FCS. During stimulation with oxytocin, the fatty concentrations of fatty acids decreased significantly in a dose-dependent manner. These results suggest that both F and P are implicated as regulatory factors in the activation of arachidonic acid cascade.
糖皮质激素可能通过抑制磷脂中花生四烯酸的脱酰作用，在几种细胞类型中抑制前列腺素的合成。我们研究了皮质醇（F）和孕酮（P）对培养的人子宫肌层细胞脂肪酸释放的影响。将子宫肌层细胞的汇合单层培养物在含类固醇的培养基中培养24小时，然后测定细胞内花生四烯酸和棕榈酸的浓度。在存在胎牛血清（FCS）的情况下，添加10^(-7)M F后，棕榈酸浓度显著降低。在不存在FCS的情况下，添加F后，两种脂肪酸的浓度均显著增加。在催产素刺激期间，添加F后花生四烯酸浓度没有变化，但棕榈酸浓度略有下降。对P进行了类似的评估。在存在FCS的情况下，添加10^(-5)M P后，棕榈酸浓度略有下降，但在不存在FCS的情况下显著增加。在催产素刺激期间，脂肪酸浓度以剂量依赖的方式显著降低。这些结果表明，F和P均作为花生四烯酸级联激活的调节因子发挥作用。
