Kidoguchi K, Ogawa M, Karam J D, McNeil J S, Fitch M S
Blood. 1979 Mar;53(3):519-22.
We cultured human umbilical cord blood erythropoietic precursors in methyl cellulose clonal assay and analyzed the synthetic rates of Hb A and Hb F in individual erythropoietic bursts. Hemoglobin was labeled with 14C-amino acids in culture, separated by slab gel isoelectric focusing techniques, and quantitated by fluorographic methods. Almost all bursts exhibited both Hb A and Hb F in varying ratios. Frequencies of the individual bursts differing in percentage Hb F biosynthesis had normal distributions. Natal erythropoietic precursors appeared to be randomly committed to Hb F synthesis.
我们在甲基纤维素克隆试验中培养人脐带血红细胞生成前体细胞，并分析单个红细胞生成集落中Hb A和Hb F的合成率。在培养过程中用14C -氨基酸标记血红蛋白，通过平板凝胶等电聚焦技术进行分离，并用荧光自显影法进行定量。几乎所有集落都以不同比例同时呈现Hb A和Hb F。Hb F生物合成百分比不同的单个集落的频率呈正态分布。出生时的红细胞生成前体细胞似乎随机地进行Hb F合成。
