García B A, Soares Barata J M, Blanco A
Cátedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
J Med Entomol. 1995 Mar;32(2):126-33. doi: 10.1093/jmedent/32.2.126.
Allozyme variability in populations of the Chagas's disease vector Triatoma infestans (Klug) was investigated by means of starch gel electrophoresis. Samples were taken from nine laboratory colonies established with individuals collected at different localities across the range of this insect in South America. Zymograms for proteins coded by a total of 17 loci were obtained. Allele frequencies, proportion of polymorphic loci (P), mean heterozygosity per locus (H), similarity (S), and identity (I) indices, genetic distance (D), and gene flow among populations were estimated. Mean values for P = 58.53% and for H = 0.095, indicating an important level of genetic variability. There was remarkable similarity among the colonies (mean I = 0.9946). Estimated gene flow among populations was high. However, on the basis of the known natural history of T. infestans, the uniformity of allele frequencies among populations may be interpreted as the result of the recent and rapid dispersal of the species from the site of origin in the Cochabamba Valley, Bolivia.
通过淀粉凝胶电泳法研究了恰加斯病媒介昆虫——骚扰锥蝽（Triatoma infestans (Klug)）种群中的等位酶变异性。样本取自九个实验室种群，这些种群是用在南美洲该昆虫分布范围内不同地点采集的个体建立起来的。共获得了由17个基因座编码的蛋白质的酶谱图。估计了等位基因频率、多态位点比例（P）、每个基因座的平均杂合度（H）、相似性（S）和同一性（I）指数、遗传距离（D）以及种群间的基因流。P的平均值为58.53%，H的平均值为0.095，表明存在重要程度的遗传变异性。各种群间存在显著相似性（平均I = 0.9946）。估计的种群间基因流较高。然而，根据已知的骚扰锥蝽自然史，种群间等位基因频率的一致性可解释为该物种近期从玻利维亚科恰班巴山谷的起源地迅速扩散的结果。
