García B A, Canale D M, Blanco A
Cátedra de Química Biológica, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Argentina.
J Med Entomol. 1995 Mar;32(2):134-7. doi: 10.1093/jmedent/32.2.134.
The genetic structure of Triatoma guasayana Wygodzinsky & Abalos, T. sordida (Stål), T. platensis Neiva, and T. infestans (Klug) was compared by starch gel electrophoresis. In total, 17 enzyme loci were analyzed in T. infestans and 14 loci in the other three species. Zymograms were useful in species identification. Icdh-2 and Hk-1 are diagnostic loci, and Icdh-1, Hk-2, Es-1, and Es-2 are helpful in distinguishing these species. The proportion of polymorphic loci for the four species (range, 52.9 to 58.3%) was somewhat higher than that expected from reports for other insects. Mean heterozygosity per locus (range, 0.062 to 0.156) was within the range reported for insects in the literature. The level of heterozygosity was highest for T. guasayana and lowest for T. sordida. Values of genetic distance (Nei's D), identity (Nei's I), and similarity (Rogers's S) indices indicate that the four species are well-defined taxa. T. infestans and T. platensis are the closest (D = 0.45, S = 0.62). T. guasayana and T. sordida (D = 0.77, S = 0.46) form another pair of related species.
通过淀粉凝胶电泳比较了瓜萨亚纳锥蝽（Triatoma guasayana Wygodzinsky & Abalos）、污色锥蝽（T. sordida (Stål)）、普拉滕斯锥蝽（T. platensis Neiva）和骚扰锥蝽（T. infestans (Klug)）的遗传结构。总共对骚扰锥蝽的17个酶位点和其他三个物种的14个位点进行了分析。酶谱图有助于物种鉴定。异柠檬酸脱氢酶-2（Icdh-2）和己糖激酶-1（Hk-1）是诊断位点，而异柠檬酸脱氢酶-1（Icdh-1）、己糖激酶-2（Hk-2）、酯酶-1（Es-1）和酯酶-2（Es-2）有助于区分这些物种。这四个物种的多态位点比例（范围为52.9%至58.3%）略高于其他昆虫报道的预期比例。每个位点的平均杂合度（范围为0.062至0.156）在文献报道的昆虫范围内。杂合度水平以瓜萨亚纳锥蝽最高，污色锥蝽最低。遗传距离（内氏D）、同一性（内氏I）和相似性（罗杰斯S）指数的值表明这四个物种是界限分明的分类单元。骚扰锥蝽和普拉滕斯锥蝽关系最近（D = 0.45，S = 0.62）。瓜萨亚纳锥蝽和污色锥蝽（D = 0.77，S = 0.46）构成另一对相关物种。
