钾离子去极化作用下大鼠离体视网膜释放[3H]-乙酰胆碱：对高亲和力胆碱摄取的依赖性
Release of [3H]-acetylcholine from the isolated retina of the rat by potassium depolarization: dependence on high affinity choline uptake.
作者信息
Massey S C, Neal M J
出版信息
Br J Pharmacol. 1979 Feb;65(2):271-6. doi: 10.1111/j.1476-5381.1979.tb07828.x.
Abstract
- The effect of potassium depolarization on the release of [3H]-acetylcholine ([3H]-ACh) from the isolated retina of the rat was studied. 2. Exposure of retinae to medium containing KCl (50 mM) evoked a large increase in the efflux of [3H]-ACh with only a small concurrent increase in the efflux of [3H]-choline. The KCl-evoked release of [3H]-ACh was almost abolished in calcium-free medium. 3. Incubation of retinae with [3H]-choline in sodium-free medium, or medium containing hemicholinum-3 (HC-3), procedures that are believed to inhibit selectively the high affinity choline transport system, reduced the retinal uptake of [3H]-choline by approximately 50% and the synthesis of [3H]-ACh by about 97%. 4. The potassium-evoked release of [3H]-ACh was almost abolished in retinae that had been loaded with [3H]-choline in sodium-free medium or medium containing HC-3, and subsequently superfused in normal medium. 5. It is suggested that as in other areas of the nervous system, a sodium-dependent, high affinity uptake system for choline is important in retinal cholinergic nerve terminals.
摘要
- 研究了钾离子去极化对大鼠离体视网膜释放[3H]-乙酰胆碱（[3H]-ACh）的影响。2. 将视网膜暴露于含氯化钾（50 mM）的培养基中，可引起[3H]-ACh外流大幅增加，而[3H]-胆碱外流仅同时小幅增加。在无钙培养基中，氯化钾诱发的[3H]-ACh释放几乎完全被抑制。3. 在无钠培养基或含半胱氨酸-3（HC-3）的培养基中用[3H]-胆碱孵育视网膜，这些方法被认为可选择性抑制高亲和力胆碱转运系统，使视网膜对[3H]-胆碱的摄取减少约50%，[3H]-ACh的合成减少约97%。4. 在无钠培养基或含HC-3的培养基中用[3H]-胆碱加载视网膜，随后在正常培养基中灌流，钾离子诱发的[3H]-ACh释放几乎完全被抑制。5. 提示与神经系统的其他区域一样，胆碱的钠依赖性高亲和力摄取系统在视网膜胆碱能神经末梢中很重要。
相似文献
1
Release of [3H]-acetylcholine from the isolated retina of the rat by potassium depolarization: dependence on high affinity choline uptake.钾离子去极化作用下大鼠离体视网膜释放[3H]-乙酰胆碱：对高亲和力胆碱摄取的依赖性
Br J Pharmacol. 1979 Feb;65(2):271-6. doi: 10.1111/j.1476-5381.1979.tb07828.x.
2
Effect of potassium depolarization and preganglionic nerve stimulation on the metabolism of [3H]-choline in rat isolated sympathetic ganglia.钾离子去极化和节前神经刺激对大鼠离体交感神经节中[3H] - 胆碱代谢的影响。
Br J Pharmacol. 1982 Dec;77(4):581-90. doi: 10.1111/j.1476-5381.1982.tb09335.x.
3
Potassium activation of [3H]-choline accumulation by isolated sympathetic ganglia of the rat.钾离子对大鼠离体交感神经节[³H] - 胆碱积累的激活作用。
Br J Pharmacol. 1982 Dec;77(4):573-80. doi: 10.1111/j.1476-5381.1982.tb09334.x.
4
Relationships between choline uptake, acetylcholine synthesis and acetylcholine release in isolated rat atria.离体大鼠心房中胆碱摄取、乙酰胆碱合成与乙酰胆碱释放之间的关系。
J Pharmacol Exp Ther. 1983 Aug;226(2):343-8.
5
Inhibition of choline efflux results in enhanced acetylcholine synthesis and release in the guinea-pig corticocerebral synaptosomes.胆碱外流的抑制导致豚鼠大脑皮质突触体中乙酰胆碱合成和释放增加。
Neurochem Int. 1992 Feb;20(2):219-27. doi: 10.1016/0197-0186(92)90171-m.
6
Age-related changes in NMDA-induced [3H]acetylcholine release from brain slices of senescence-accelerated mouse.衰老加速小鼠脑片NMDA诱导的[3H]乙酰胆碱释放的年龄相关变化。
Int J Dev Neurosci. 1992 Apr;10(2):121-9. doi: 10.1016/0736-5748(92)90040-7.
7
Surplus acetylcholine and acetylcholine release in the rat diaphragm.大鼠膈肌中多余的乙酰胆碱及乙酰胆碱释放
J Physiol. 1987 Apr;385:147-67. doi: 10.1113/jphysiol.1987.sp016489.
8
Regulation of synaptic acetylcholine concentrations by acetylcholine transport in rat striatal cholinergic transmission.大鼠纹状体胆碱能传递中乙酰胆碱转运对突触乙酰胆碱浓度的调节
J Neurochem. 2017 Oct;143(1):76-86. doi: 10.1111/jnc.14127. Epub 2017 Aug 18.
9
Effect of stimulation with light on synthesis and release of acetylcholine by an isolated mammalian retina.光刺激对离体哺乳动物视网膜乙酰胆碱合成与释放的影响。
J Neurophysiol. 1976 Nov;39(6):1210-9. doi: 10.1152/jn.1976.39.6.1210.
10
The synthesis and release of acetylcholine in normal and denervated rat diaphragms during incubation in vitro.在体外孵育过程中正常和去神经大鼠膈肌中乙酰胆碱的合成与释放。
J Physiol. 1983 Jan;334:461-74. doi: 10.1113/jphysiol.1983.sp014506.
引用本文的文献
1
Intrinsic Expression of Coagulation Factors and Protease Activated Receptor 1 (PAR1) in Photoreceptors and Inner Retinal Layers.感光细胞和视网膜内层固有凝血因子和蛋白酶激活受体 1（PAR1）的表达。
Int J Mol Sci. 2022 Jan 17;23(2):984. doi: 10.3390/ijms23020984.
2
P2X receptors supply extracellular choline as a substrate for acetylcholine synthesis.P2X 受体提供细胞外胆碱作为乙酰胆碱合成的底物。
FEBS Open Bio. 2022 Jan;12(1):250-257. doi: 10.1002/2211-5463.13332. Epub 2021 Nov 27.
3
Acetylcholine induces GABA release onto rod bipolar cells through heteromeric nicotinic receptors expressed in A17 amacrine cells.乙酰胆碱通过A17无长突细胞中表达的异聚烟碱受体诱导GABA释放到视杆双极细胞上。
Front Cell Neurosci. 2015 Feb 9;9:6. doi: 10.3389/fncel.2015.00006. eCollection 2015.
4
Role of ACh-GABA cotransmission in detecting image motion and motion direction.乙酰胆碱-γ-氨基丁酸共传递在检测图像运动和运动方向中的作用。
Neuron. 2010 Dec 22;68(6):1159-72. doi: 10.1016/j.neuron.2010.11.031.
5
On the mechanism by which veratridine causes a calcium-independent release of gamma-aminobutyric acid from brain slices.关于藜芦碱导致脑片在不依赖钙的情况下释放γ-氨基丁酸的机制。
Br J Pharmacol. 1981 Jul;73(3):655-67. doi: 10.1111/j.1476-5381.1981.tb16801.x.
6
Effect of excitatory amino acids and analogues on [3H]acetylcholine release from amacrine cells of the rabbit retina.兴奋性氨基酸及其类似物对兔视网膜无长突细胞释放[3H]乙酰胆碱的影响。
J Physiol. 1985 Sep;366:47-62. doi: 10.1113/jphysiol.1985.sp015784.
本文引用的文献
1
Electrophoresis of acetylcholine, choline and related compounds.乙酰胆碱、胆碱及相关化合物的电泳
Biochem Pharmacol. 1967 Jul 7;16(7):1386-8. doi: 10.1016/0006-2952(67)90174-8.
2
An enzyme assay for acetylcholine.一种用于检测乙酰胆碱的酶测定法。
Biochem Pharmacol. 1969 Jun;18(6):1479-86. doi: 10.1016/0006-2952(69)90262-7.
3
Release of acetylcholine from hippocampal slices by potassium depolarization: dependence on high affinity choline uptake.钾离子去极化诱导海马切片释放乙酰胆碱：对高亲和力胆碱摄取的依赖性。
Brain Res. 1974 Apr 19;70(2):372-6. doi: 10.1016/0006-8993(74)90329-1.
4
High affinity transport of choline into synaptosomes of rat brain.胆碱向大鼠脑突触体的高亲和力转运。
J Neurochem. 1973 Dec;21(6):1355-74. doi: 10.1111/j.1471-4159.1973.tb06022.x.
5
Choline: selective accumulation by central cholinergic neurons.胆碱：由中枢胆碱能神经元选择性积累。
J Neurochem. 1973 Feb;20(2):581-93. doi: 10.1111/j.1471-4159.1973.tb12157.x.
6
Comparison of the localization of acetylcholinesterase and non-specific cholinesterase activities in mammalian and avian retinas.哺乳动物和鸟类视网膜中乙酰胆碱酯酶与非特异性胆碱酯酶活性定位的比较。
J Comp Neurol. 1968 May;133(1):1-16. doi: 10.1002/cne.901330102.
7
Further evidence for cholinergic habenulo-interpeduncular neurons: pharmacologic and functional characteristics.胆碱能缰核-脚间核神经元的进一步证据：药理学和功能特征
Brain Res. 1975 Oct 31;97(2):265-75. doi: 10.1016/0006-8993(75)90449-7.
8
High-affinity choline transport in the isolated retina.离体视网膜中的高亲和力胆碱转运
Brain Res. 1975 Aug 15;93(3):548-51. doi: 10.1016/0006-8993(75)90197-3.
9
Responses to acetylcholine of ganglion cells in an isolated mammalian retina.分离的哺乳动物视网膜中神经节细胞对乙酰胆碱的反应。
J Neurophysiol. 1976 Nov;39(6):1220-35. doi: 10.1152/jn.1976.39.6.1220.
10
Effect of stimulation with light on synthesis and release of acetylcholine by an isolated mammalian retina.光刺激对离体哺乳动物视网膜乙酰胆碱合成与释放的影响。
J Neurophysiol. 1976 Nov;39(6):1210-9. doi: 10.1152/jn.1976.39.6.1210.
Zotero 插件