Wiggins R C, Fuller G N
Brain Res. 1979 Feb 16;162(1):103-12. doi: 10.1016/0006-8993(79)90759-5.
We used a double isotope procedure and starved and normal littermate rats to compare relative protein synthesis in the cerebellar nuclear, myelin, synaptosomal, mitochondrial, and microsomal subfractions of postnatally starved animals. The remaining brain tissue was dissected into 6 additional regions (cerebral cortex, medulla oblongata, midbrain, hippocampus, striatum, and hypothalamus) and these were frozen for similar subcellular fractionation and analysis at a later date. The microsomal fraction derived from frozen tissues was discarded. The results show that early postnatal starvation specifically depresses myelin synthesis to about the same extent in all major brain regions at 18 and 21 days of age.
我们采用双同位素程序，对饥饿的和正常的同窝出生大鼠进行实验，以比较出生后饥饿动物的小脑核、髓磷脂、突触体、线粒体和微粒体亚组分中的相对蛋白质合成情况。其余脑组织被解剖为另外6个区域（大脑皮层、延髓、中脑、海马体、纹状体和下丘脑），并将这些区域冷冻起来，以便日后进行类似的亚细胞分级分离和分析。来自冷冻组织的微粒体组分被丢弃。结果表明，出生后早期饥饿在18日龄和21日龄时，会使所有主要脑区的髓磷脂合成均受到特异性抑制，且抑制程度大致相同。
