Kosaka K, Yamanobe S, Tomiyama S, Yagi T
Department of Otorhinolaryngology, Nippon Medical School, Tokyo, Japan.
Acta Otolaryngol Suppl. 1995;519:176-7. doi: 10.3109/00016489509121897.
In order to identify the inner ear autoantibodies, sera of 195 patients with sensorineural hearing loss and/or vertigo were reached with bovine inner ear antigen by western blotting method. Positive reaction was seen at several molecular weights, in which 33-35 kD, 42 kD and 68 kD were highly reactive. In moderate to severe hearing loss patients, positive reactions with 33-35 kD, 42 kD, 68 kD and others were 25.8%, 26.8%, 26.8% and 71.5% respectively. On the other hand, a positive rate did not clearly correlate with diagnostic entities. Further study to purify inner ear antigen may evaluate inner ear autoimmune disease.
为了鉴定内耳自身抗体，采用蛋白质印迹法，用牛内耳抗原检测了195例感音神经性听力损失和/或眩晕患者的血清。在几个分子量处出现阳性反应，其中33 - 35kD、42kD和68kD反应性较高。在中度至重度听力损失患者中，33 - 35kD、42kD、68kD及其他分子量的阳性反应率分别为25.8%、26.8%、26.8%和71.5%。另一方面，阳性率与诊断实体无明显相关性。进一步纯化内耳抗原的研究可能有助于评估内耳自身免疫性疾病。
