Gabbai F B, Thomson S C, Peterson O, Wead L, Malvey K, Blantz R C
Division of Nephrology-Hypertension, Veterans Affairs Medical Center, San Diego, California, USA.
Am J Physiol. 1995 Jun;268(6 Pt 2):F1004-8. doi: 10.1152/ajprenal.1995.268.6.F1004.
Endothelium-dependent nitric oxide (EDNO) exerts control over the processes of glomerular filtration and tubular reabsorption. The importance of the renal nerves to the tonic influence of EDNO in the glomerular microcirculation and proximal tubule was tested by renal micropuncture in euvolemic adult male Munich-Wistar rats. The physical determinants of glomerular filtration and proximal reabsorption were assessed before and during administration of the nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (L-NMMA), in control animals and in animals 5-9 days after either ipsilateral surgical renal denervation (DNX) or after either sham surgery (SHX). L-NMMA caused single-nephron glomerular filtration rate to decline in control and SHX animals but not in DNX rats. L-NMMA caused a reduction in proximal reabsorption in control and SHX rats, which was prevented by prior DNX. DNX did not alter urinary guanosine 3',5'-cyclic monophosphate excretion, and, although DNX upregulates glomerular angiotensin II (ANG II) receptors, prior DNX did not alter intrarenal ANG II content as evaluated by radioimmunoassay. Some component of renal adrenergic activity is required for the full expression of the glomerular and tubular effects of blockade of nitric oxide synthase.
内皮依赖性一氧化氮（EDNO）对肾小球滤过和肾小管重吸收过程发挥着调控作用。通过对成年雄性正常血容量的慕尼黑-维斯塔大鼠进行肾脏微穿刺，测试了肾神经对EDNO在肾小球微循环和近端小管中紧张性影响的重要性。在对照动物以及同侧手术去肾神经支配（DNX）或假手术（SHX）5 - 9天后的动物中，在给予一氧化氮合酶抑制剂NG-单甲基-L-精氨酸（L-NMMA）之前和期间，评估了肾小球滤过和近端重吸收的物理决定因素。L-NMMA导致对照动物和SHX动物的单肾单位肾小球滤过率下降，但在DNX大鼠中未出现这种情况。L-NMMA导致对照大鼠和SHX大鼠近端重吸收减少，而预先进行DNX可防止这种情况。DNX并未改变尿中鸟苷3',5'-环磷酸的排泄，并且，尽管DNX上调了肾小球血管紧张素II（ANG II）受体，但通过放射免疫测定评估，预先进行DNX并未改变肾内ANG II含量。一氧化氮合酶阻断对肾小球和肾小管的影响得以充分表达需要肾肾上腺素能活性的某些成分参与。
