Pasternak G W, Pan Y X, Cheng J
Cotzias Laboratory of Neuro-Oncology Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Ann N Y Acad Sci. 1995 May 10;757:332-8. doi: 10.1111/j.1749-6632.1995.tb17491.x.
We cloned a kappa 3-related opioid receptor, and although it is still not clear whether this clone corresponds to the kappa 3 receptor itself or is a related gene product, the extensive antisense mapping and the antibody immunoreactivity strongly associate this clone with the kappa 3 receptor. Our approach also indicates the usefulness of antisense approaches in mapping and identifying orphan receptors. Perhaps it is most effective in identifying partial sequences prior to cloning them in their entirety. It also provides a mechanism of identifying proteins that are not expressed functionally.
我们克隆了一种与κ3相关的阿片受体，尽管目前尚不清楚该克隆是否对应于κ3受体本身或是否为相关基因产物，但广泛的反义图谱和抗体免疫反应性强烈表明该克隆与κ3受体相关。我们的方法还表明了反义方法在绘制和鉴定孤儿受体方面的有用性。也许它在完整克隆之前识别部分序列时最为有效。它还提供了一种识别未功能性表达的蛋白质的机制。
