Saiki I, Une Y, Uchino J
First Dept. of Surgery, Hokkaido University School of Medicine.
Gan To Kagaku Ryoho. 1995 Jun;22 Suppl 2:103-9.
Nuclear DNA ploidy analysis, p53 immunohistochemical overexpression and PCNA Labeling Index (PCNA LI) were studied in 80 cases of resected "single nodular" human hepatocellular carcinoma (HCC) tissue sections. Aneuploid pattern was found in forty-six cases (57.7%) and p53 overexpression in fifteen cases (18.8%). The rate of aneuploid pattern was significantly higher in patients with carcinomas more than 2 cm in diameter, fc-inf positive growth and more than Stage II. p53 overexpression was not found in patients with well-differentiated HCCs and Stage I. Positive rate of p53 overexpression was significantly higher in patients with HCV-Ab positive and PCNA LI > or = 40%. It was high in patients with vp-positive, vv-positive and aneuploid pattern. PCNA LI of HCCs were significantly higher in patients with HBs-Ag positive, high serum AFP level, massive type and single nodular type with proliferation into surrounding area, fc-inf positive, im-positive, Stage III + IV, aneuploid pattern and p53 overexpression. The postoperative prognoses of patients with aneuploid pattern and PCNA LI > or = 40% were significantly poorer than those of the diploid one and PCNA LI < 40% in cumulative survival rates. Those prognoses were remarkably poorer in the early postoperative period. Patients with p53 overexpression had poorer prognosis than those with no p53 overexpression in the early postoperative period. These results suggest that nuclear DNA ploidy analysis, p53 immunohistochemical overexpression and PCNA LI were useful markers of biological malignant potentials in "single nodular" human HCCs.
对80例手术切除的“单结节”人肝细胞癌（HCC）组织切片进行了核DNA倍体分析、p53免疫组化过表达及增殖细胞核抗原标记指数（PCNA LI）研究。46例（57.7%）呈现非整倍体模式，15例（18.8%）有p53过表达。直径大于2cm、fc-inf阳性生长及II期以上的癌患者中，非整倍体模式发生率显著更高。高分化HCC及I期患者未发现p53过表达。HCV-Ab阳性且PCNA LI≥40%的患者中，p53过表达阳性率显著更高。vp阳性、vv阳性及非整倍体模式的患者中该阳性率较高。HBs-Ag阳性、血清甲胎蛋白水平高、巨块型及向周围区域浸润性生长的单结节型、fc-inf阳性、im阳性、III + IV期、非整倍体模式及p53过表达的HCC患者，其PCNA LI显著更高。非整倍体模式且PCNA LI≥40%的患者术后累积生存率的预后明显比二倍体模式且PCNA LI＜40%的患者差。术后早期这些预后明显更差。术后早期p53过表达的患者比无p53过表达的患者预后更差。这些结果表明，核DNA倍体分析、p53免疫组化过表达及PCNA LI是“单结节”人HCC生物学恶性潜能的有用标志物。
